Zogulitsa

APS-5 ndi gawo la chemiluminescent lochokera ku 9,10-dihydroacridine, lomwe limagwiritsidwa ntchito makamaka kuti ELISA izindikire mankhwala omwe ali ndi alkaline phosphatase AP conjugates;9,10-dihydroacridine mu kapangidwe ka APS-5 The hydroacridine dongosolo akhoza zimatulutsa kuwala mosalekeza, stably ndi efficiently pansi pa zochita za AP, ndi APS-5 ndi abwino kwambiri phosphatase ntchito njira assay ndi phosphatase enzyme-zogwirizana immunoassay.

Monga kafukufuku wowunikira, amagwiritsidwa ntchito pophunzira tchipisi ta jini.Zomwe zimachitika zimatchedwa acridan (9,10-dihydroacridine) ngati gawo lapansi ndi zamchere phosphatase, zomwe zimapangitsa kuti chemiluminescence ikhale yolimba kwambiri.Amapereka kukhudzika kwapamwamba komanso kosavuta kugwiritsa ntchito alkaline phosphatase conjugation pakuzindikira kwa chemiluminescent.Alkaline phosphatase imazindikiridwa mochepera 10-19 mol, imakwera kwambiri kuti ichepetse nthawi yodziwikiratu ndikuwonjezera kutulutsa, ndipo kutsetsereka kwa mzere wokhotakhota kumapangidwa ndi logarithmic yofanana ndi 1.0.Kuchuluka kumodzi kapena kupitilira kwa enzyme kumatulutsa kuchuluka kwa luminescence kapena kupitilira apo, kuwala kosalekeza - osafuna nthawi yoyeserera.Kuchuluka kwa luminescence kumatha kuwerengedwa kuchokera pamapindikira owongolera omwe amapangidwa nthawi iliyonse, ndipo zotsatira zake sizimamva kutentha kwapakati pa 22 ° C - 35 ° C, kumachepetsa kulondola komwe kumafunikira kuwongolera kutentha.

ubwino:

1 Kukhudzika kwakukulu - zolembera zamapuloteni pansi pa 5pg zitha kudziwika;

2 Kuwala kowala kwambiri - nsonga yowala imatha kufikira kwakanthawi kochepa;

3 Luminescence yopitilira komanso yokhazikika - njira yake yowunikira simakhudzidwa ndi kutentha mkati mwa 25-35 ℃, ndipo sikufuna kuwongolera kutentha.

Kugwiritsa ntchito kuyerekeza kwa magawo omwe amagwiritsidwa ntchito nthawi zambiri a luminescent APS-5 (193884-53-6) ndi AMPPD (122341-56-4)

Onse APS-5 (193884-53-6) ndi AMPPD (122341-56-4) ndi magawo a chemiluminescent a alkaline phosphatase, ndipo onsewa amatchedwa ultra-high sensitivity kuzindikira reagents, kotero kuti ma reagents awiriwa ali mu kuzindikira Zomwe zili bwino komanso choyipa nchiyani?Kodi kusankha pamene ntchito?

Kumbali imodzi, APS-5 ikagwiritsidwa ntchito ngati gawo la alkaline phosphatase kuzindikira gawo lapansi, chidziwitso chodziwikiratu chimatha kufikira 10-19 molar ndende, nthawi yachitukuko chamtundu imatha kufika pachimake pafupifupi masekondi 10, ndipo fulorosenti imatha kwa nthawi yayitali. nthawi.Mwa izi, APS 5 ikhoza kunenedwa kuti ili ndi katundu wabwino kwambiri.Komabe, APS-5 imakhala yochepa pa kutentha kwa ntchito, ndipo kutentha kwake komwe kumapereka mtundu kumakhala pansi pa 20'C.Kumbali ina, pamene AMPPD imagwiritsidwa ntchito ngati gawo lodziwikiratu la alkaline phosphatase, chidziwitso chodziwikiratu chimatchedwanso ultra-high sensitivity, koma sichikhoza kufika pa mlingo wa APS 5. Nthawi yachitukuko cha mtundu imafika pachimake pa masekondi pafupifupi 30. , ndi nthawi ya fluorescence yaitali.AMPPD ndiyabwino kwambiri pazomwe zili pamwambazi, koma ndizotsika kwambiri ku APS5, koma kutentha kwake komwe kumapereka utoto sikovuta monga APS5, komanso kutentha kwa 2--8C ndikosavuta kuposa APS-5.Mwachidule, ngakhale APS-5 imaposa AMPPD pakuzindikira kulondola, imataya kukhazikika.Momwe mungasankhire zimatengera zosowa zenizeni zozindikirika.

1. Chiyambi cha APS-5 (193884-53-6):

Dzina lachingerezi: APS-5

Nambala ya CAS: 193884-53-6

Fomula ya maselo: C21H15ClNO4PS Na2

Kulemera kwa molekyulu: 489.819

Maonekedwe: ufa wosalala-woyera

Zomwe zili: ≥98%

Zofunika: 1g, 5g, 10g

Kusungirako zinthu: -20 ℃ kusungidwa

(4-Chlorophenylmercapto) (10-methyl-9,10-dihydroacridine methylene) mchere wa phosphate disodium, wotchedwa APS-5, ndi reagent wa chemiluminescent ndipo umagwiritsidwa ntchito ngati gawo lapansi la chemiluminescent.Amapereka kukhudzika kwapamwamba komanso kosavuta kugwiritsa ntchito alkaline phosphatase conjugation pakuzindikira kwa chemiluminescent.Zomwe zimachitika zimatchedwa acridan (9,10-dihydroacridine) ngati gawo lapansi ndi zamchere phosphatase, zomwe zimapangitsa kuti chemiluminescence ikhale yolimba kwambiri.APS-5 ndi njira yabwino yodziwira ntchito ya phosphatase poyesa mayankho ndi ma immunoassays okhudzana ndi phosphatase enzyme.Monga gawo laling'ono lowala, APS-5 ili ndi chidwi chodziwikiratu komanso nthawi yayifupi yochita.

2. Kufotokozera mwatsatanetsatane kwa AMPPD (122341-56-4)

Dzina lachingerezi: AMPPD

Nambala ya CAS: 122341-56-4

Fomula ya maselo: C18H23O7P

Kulemera kwa molekyulu: 382.344781

Maonekedwe: ufa woyera

Chiyero: ≥97%

Zofunika: 1g, 5g, 10g

Kusungirako zinthu: -20 ℃ kusungidwa

Chenjezo: Iyenera kukhala youma kupewa pafupipafupi Kutha ndi lyophilization