Zogulitsa

Kuletsa kwa β-carbonic anhydrases (CAs, EC 4.2.1.1) kuchokera ku tizilombo toyambitsa matenda Cryptococcus neoformans (Can2) ndi Candida albicans (Nce103) ndi mndandanda wa 25 nthambi za aliphatic ndi zonunkhira za carboxylate zafufuzidwa.Ma isoforms aumunthu hCA I ndi II adaphatikizidwanso mu phunziroli kuti afanizidwe.Aliphatic carboxylates nthawi zambiri anali millimolar hCA I ndi II inhibitors ndi otsika micromolar/submicromolar β-CA inhibitors.Ma carboxylates onunkhira anali ma micromolar inhibitors a michere inayi koma ena mwa iwo adawonetsa zochitika zochepa za nanomolar motsutsana ndi mafangasi a pathogenic.4-Hydroxy- ndi 4-methoxy-benzoate yoletsedwa Can2 yokhala ndi K(I)s ya 9.5-9.9 nM.Ma methyl esters, hydroxamates, hydrazides ndi carboxamides a zina mwazochokera izi analinso zoletsa zogwira ntchito za α- ndi β-CAs adafufuzidwa apa.

Ma Parabens ndi ena mwa zoteteza zomwe zimagwiritsidwa ntchito mobwerezabwereza kuletsa kukula kwa tizilombo tating'onoting'ono ndikukulitsa moyo wa alumali wazinthu zosiyanasiyana zogula.Cholinga cha phunziroli chinali kupeza chidziwitso cha kagayidwe ka parabens m'maselo a khansa ya m'mawere (MCF7) popeza awonetsa zochitika za estrogenic ku maselowa ndipo apezeka m'magulu a khansa ya m'mawere.Kuopsa kwa ma parabens ku maselo a MCF7 kunatsimikiziridwa pogwiritsa ntchito mayeso a MTT.Hydrolysis ya methyl-, butyl ndi benzyl-paraben to p-hydroxybenzoic acid idawunikidwa m'maselo otukuka a MCF7 komanso ma homogenates am'manja.Glucuronidation ndi sulfoconjugation adaphunzira mu MCF7 homogenates, ndipo parabens adawunikidwa ndi HPLC.Methyl-paraben adawonetsedwa kuti ndi oopsa kwambiri kuposa butyl ndi benzyl-paraben.Ma Parabens anali okhazikika kwathunthu mu MCF7 homogenates pomwe p-nitrophenyl acetate, mtundu wa gawo lapansi, adakumana ndi hydrolysis.Ma cell a MCF7 homogenates sanafotokoze ntchito za glucuronidation ndi sulfoconjugation kwa parabens.Kukhazikika kwapamwamba kwa ma parabens kumatha kufotokozera kudzikundikira kwawo mu minofu ya khansa ya m'mawere.