Zogulitsa

1.Kufufuza ngati adenosine diphosphate (ADP) -derived adenosine ingalepheretse kuphatikizika kwa mapulateleti, makamaka pamaso pa mdani wa P2Y₁₂, kumene zotsatira za ADP pa P2Y₁₂ receptor zingalephereke. peptide mwa kuwerengera mapulateleti mu plasma (PRP) ndi magazi athunthu pamaso pa ADP ndi P2Y₁₂ antagonists cangrelor, prasugrel yogwira metabolite, ndi ticagrelor.Pamaso pa wotsutsa wa P2Y₁₂, preincubation ya PRP ndi ADP inhibited aggregation;Izi zidathetsedwa ndi adenosine deaminase.Palibe kuletsa kuphatikizika komwe kudachitika m'magazi athunthu kupatula pamene dipyridamole idawonjezedwa kuletsa kulowetsedwa kwa adenosine mu erythrocytes.Zotsatira za ADP mu PRP ndi magazi athunthu zinasinthidwanso pogwiritsa ntchito adenosine ndipo zinali zogwirizana mwachindunji ndi kusintha kwa cAMP (kuyesedwa ndi vasodilator-stimulated phosphoprotein phosphorylation).Zotsatira zonse zinali zofanana mosasamala kanthu za ant agonist a P2Y₁₂ omwe amagwiritsidwa ntchito.ADP imaletsa kuphatikizika kwa mapulateleti pamaso pa otsutsa a P2Y₁₂ kupyolera mu kutembenuka kwa adenosine.Kuletsa kumachitika mu PRP koma osati m'magazi athunthu kupatula pamene adenosine kutengako kumaletsedwa.Palibe m'modzi mwa otsutsa a P2Y₁₂ omwe adawerengedwa adatengera zotsatira za dipyridamole pazoyeserera zomwe zidachitika.

2.ADP imatengedwa kuti ndi agonist yofooka ya platelet chifukwa cha mayankho ochepa omwe amachititsa kuti mu vitro ikhale ndi thupi la extracellular Ca (2+) [(Ca (2+) )) (o) ].Kutsitsa [Ca(2+) ](o) kumawonjezera modabwitsa kuti ADP-evoked aggregation, zotsatira zake zimatheka chifukwa cha kuchulukitsidwa kwa thromboxane A(2).Kafukufukuyu adawunika ntchito ya ectonucleotidases mu [Ca (2+)] (o) -kudalira kuyambika kwa mapulateleti.Kuchepetsa [Ca(2+) ](o) kuchoka ku milimolar kupita ku milingo ya micromolar kusandulika ADP (10 μmol/l) -kudzutsa kuphatikizika kwa mapulateleti kuchoka kunthawi yochepa kupita ku kuyankhidwa kosalekeza m'madzi a m'magazi okhala ndi mapulateleti ambiri komanso kuyimitsidwa kosamba.Kutsekereza thromboxane A(2) kupanga ndi aspirin sikunakhudze izi [Ca(2+) ](o) -kudalira.Kupewa kuwonongeka kwa ADP kunathetsa kusiyana pakati pa otsika ndi okhudza thupi [Ca(2+) ](o) zomwe zimapangitsa kuti pakhale kugwirizanitsa mwamphamvu ndi kosalekeza m'mikhalidwe yonseyi.Kuyeza kwa ADP ya extracellular kunavumbulutsa kuchepa kwa plasma ndi apyrase-containin g saline pa micromolar poyerekeza ndi millimolar [Ca (2+) ](o) .Monga tanena kale, m'badwo wa thromboxane A(2) udakulitsidwa pang'onopang'ono [Ca(2+)](o) , komabe izi zinali zodziyimira pawokha ndi ectonucleotidase ntchito(.) P2Y receptor antagonists cangrelor ndi MRS2179 adawonetsa kufunikira kwa P2Y(12) zolandilira pakuphatikiza kokhazikika kwa ADP, yokhala ndi gawo laling'ono la P2Y(1) .Pomaliza, Ca (2+) -odalira ectonucleotidase ntchito ndi chinthu chachikulu chomwe chimatsimikizira kuchuluka kwa mapulateleti ku ADP ndipo chiyenera kuyendetsedwa mu maphunziro a P2Y receptor activation.