Zogulitsa

Mbiri ndi zolinga: Molybdenum (Mo) ndi chinthu chofunikira kwambiri chotsata mbewu zapamwamba.Zasonyezedwa kuti kugwiritsa ntchito Mo kumawonjezera kuzizira kwa tirigu wa dzinja.Kuti timvetsetse bwino njira zamamolekyulu zolimbana ndi kuzizira kobwera chifukwa chogwiritsa ntchito tirigu wa Mo m'nyengo yozizira, kafukufuku adapangidwa okhudzana ndi kulembedwa kwa majini oyankha mozizira (COR) munjira zodalira abscisic acid (ABA) komanso zodziyimira pawokha za ABA mu tirigu wachisanu woyendetsedwa ndi Mo application pansi pa kupsinjika kwa kutentha.

Njira: Mitundu iwiri ya tirigu wa dzinja (Triticum aestivum), cultivar Mo-efficient cultivar '97003' ndi Mo-inefficient cultivar '97014', idalimidwa mowongolera (-Mo) ndi feteleza wa Mo (+Mo) kwa 40 d pa 15/12 Madigiri C (usana/usiku), ndipo kutentha kunachepetsedwa kufika 5/2 digiri C (usana/usiku) kuti apange kupsinjika kwa kutentha kochepa.Zochita za Aldehyde oxidase (AO), zomwe zili mu ABA, zolembedwa zamtundu wa leucine zipper (bZIP)-type transcript factor (TF), majini a COR odalira ABA, CBF/DREB transcription factor genes ndi ABA-independent COR gene adafufuzidwa pa 0 , 3, 6 ndi 48 h pambuyo pa kuzizira kozizira.

Zotsatira zazikulu: Kugwiritsa ntchito kwa Mo kunachulukitsa kwambiri ntchito ya AO, milingo ya ABA, ndi mawonekedwe amtundu wa bZIP-mtundu wa TF (Wlip19 ndi Wabi5) ndi majini a COR omwe amadalira ABA (Wrab15, Wrab17, Wrab18 ndi Wrab19).Kugwiritsa ntchito Mo kuchulukitsa mawu amtundu wa CBF/DREB transcription factor genes (TaCBF ndi Wcbf2-1) ndi ABA-yodziimira COR majini (Wcs120, Wcs19, Wcor14 ndi Wcor15) pambuyo pa 3 ndi 6 h kukhudzana ndi kutentha kochepa.

Mapeto: Mo atha kuwongolera kawonekedwe ka majini a COR omwe amadalira ABA kudzera m'njira: Mo --> AO --> ABA --> bZIP --> COR yodalira ABA mu tirigu wa dzinja.Yankho la njira yodalira ABA yopita ku Mo inali isanakwane ya njira yodziyimira payokha ya ABA.Kufanana ndi kusiyana pakati pa mitundu ya tirigu ya Mo-efficient ndi Mo-infficient poyankha Mo pansi pa kuzizira kumakambidwa.