Zogulitsa

Tet 5-methylcytosine dioxygenases imathandizira DNA demethylation popanga 5-hydroxymethylcytosine ndi zina zowonjezera okosijeni.Tet1 ndi Tet2 amawonetsedwa kwambiri m'maselo a mbewa pluripotent ndipo amachepetsedwa mosiyanasiyana m'maselo a somatic, koma njira zolembera sizikudziwika bwino.Apa tidafotokozera madera olimbikitsa ndi othandizira mu Tet1 ndi Tet2.Mukati mwa 15-kb "superenhancer" wa Tet1, pali malo awiri oyambira (TSSs) okhala ndi machitidwe osiyanasiyana panthawi ya chitukuko.Chigawo cholimbikitsa cha 6-kb kumtunda kwa distal TSS chimagwira ntchito kwambiri m'maselo osadziwika bwino, kufotokoza mozama mawu a Tet1 mu transgenic system, ndipo mofulumira amakumana ndi DNA methylation ndikuletsa kusiyana kwa maselo otukuka ndi epiblast.TSS yachiwiri kunsi kwa mtsinje, yolumikizidwa ndi wolimbikitsa wofooka kwambiri wa CpG, imayendetsedwa ndi chowonjezera choyandikana ndi ma cell a embryonic stem cell (ESCs) ndi ma cell ngati epiblast (EpiLCs).Tet2 ili ndi CpG pachilumba p romoter yokhala ndi zochita zodziyimira pawokha komanso ESC-specific distal intragenic enhancer;yotsirizirayi imachepetsedwa mofulumira mu EpiLCs.Kafukufuku wathu akuwonetsa mitundu yosiyana ya malamulo olembera ku Tet1 ndi Tet2 panthawi yakusintha kwa cell pakukula koyambirira.Atolankhani atsopano a transgenic omwe amagwiritsa ntchito madera olamulira a Tet1 ndi Tet2 atha kusiyanitsa kusintha kosasinthika m'maiko ambiri komanso kusiyanasiyana kwamtundu wa epigenetic.