Zogulitsa

Mitsempha hyperpermeability imathandizira kudwala mu kutupa.Njira zamakono zowunikira mu vivo kupenya kwamphamvu kutengera kutulutsa kwa albumin ya Evans Blue (EB) ndizovuta ndipo nthawi zambiri zimakhala zopanda chidwi.Tinapanga njira yatsopano ya infrared fluorescence (IRF) yoyezera EB-albumin extravasation kuti tidziwe kuchuluka kwa mitsempha ya mitsempha mu zitsanzo za murine.Kuthekera kwa minyewa yoyambitsidwa ndi endotoxaemia kudawunikidwa paziwalo zonse zolimba, ubongo, khungu ndi peritoneum ndi IRF komanso muyeso wachikhalidwe wa EB muzotulutsa za minofu.Organ IRF idakula motsatana ndi kuchuluka kwa mtsempha wa EB (2.5-25 mg/kg).Tissue IRF inali yovuta kwambiri pakudzikundikira kwa EB poyerekeza ndi njira yotengera kuyamwa.Momwemo, kusiyana kwa mitsempha ya mitsempha ndi EB kudzikundikira pakati pa mbewa za lipopolysaccharide-treated and saline-treated nthawi zambiri zimakhala zofunikira pamene zimafufuzidwa ndi kuzindikira kwa IRF-based koma osati chifukwa cha kuyamwa.EB idapezeka m'zigawo zonse za 353 zomwe zidawunikidwa ndi IRF koma mu 67% (239/353) ya ziwalo zomwe zidawunikidwa ndi njira yotengera kuyamwa, kuwonetsa kukhudzika kwa kuzindikira kwa EB mu ziwalo zomwe zili ndi IRF.Mosiyana ndi zimenezi, EB mu plasma pambuyo pa kayendetsedwe ka EB inayesedwa mosavuta ndi njira zonse zomwe zili ndi mgwirizano waukulu pakati pa njira ziwiri (n = 116, r2 = 0.86).Kuchulukitsidwa kwa kusiyana kwapadera kwa EB-IRF chifukwa cha endotoxin kunali koyenera pamene IRF inafanizidwa pakati pa mbewa zofananira kulemera, jenda, ndi msinkhu, komanso ndi kukonza koyenera kwa kulemera kwa chiwalo ndi EB plasma ndende.Makamaka, njira ya EB-IRF imasiya ziwalo kuti zisakhale bwino chifukwa cha histopathology yotsatira.Mwachidule, EB-IRF ndi buku, lomvera kwambiri, lachangu, komanso njira yabwino yowerengera kuchuluka kwa EB m'zigawo zochiritsira zolimbana ndi mbewa.