Zogulitsa

Kuwunika mwadongosolo mawonekedwe odetsa ndi chitetezo cha utoto watsopano womwe ungakhalepo pa opaleshoni ya intraocular. Mitundu isanu ndi umodzi idaphatikizidwa pakufufuza: kuwala kobiriwira SF (LGSF) yellowish, E68, bromophenol blue (BPB), Chicago blue (CB), rhodamine 6G, rhodulinblau -zoyambira 3 (RDB-B3).Utoto wonse unasungunuka ndi kusungunuka mumchere wa saline.Mphamvu zoyamwa kuwala kwa utoto uliwonse zidayesedwa pamlingo wa 0.05% pakati pa 200 ndi 1000 nm.Makhalidwe odetsa adawunikidwa ndi kudetsa minofu ya lens capsule ndi epiretinal nembanemba (ERMs), kuchotsedwa mosadukiza, ndikuyika utoto wa 1.0%, 0.5%, 0.2%, ndi 0.05%.Maso a nkhumba (nthawi ya postmortem, maola 9) analinso odetsedwa.Poizoni wokhudzana ndi utoto adawunikidwa ndi mayeso a colorimetric (MTT) kuyeza kuletsa kwa retinal pigment epithelium (RPE) cell proliferation (ARPE-19 ndi ma cell a RPE amunthu, ndime 3-6).Kuthekera kwa ma cell kunayesedwanso potengera kuyeserera kwa ma cell amitundu iwiri.Utoto unafufuzidwa m'magulu a 0.2% ndi 0.02%.Mitundu yonse yomwe inafufuzidwa mu phunziroli inadetsa makapisozi a lens aumunthu, amachotsedwa intraoperative;ERMs, yosenda pa opaleshoni ya macular pucker;ndi enucleated maso a nkhumba, kutengera ndende ntchito.Mayamwidwe aatali atalitali a utotowo anali mkati mwa 527 mpaka 655 nm pamlingo wa 0.05%.Rhodamine G6 ndi RDB-B3 adawonetsa zotsatira zoyipa pakukula kwa ma cell a ARPE-19 pagulu la 0.2% ndipo sanaphatikizidwe pakufufuza kwina m'maselo oyamba a RPE.Mitundu inayi yotsalayo sinawonetse zotsatirapo zoyipa pa ARPE-19 komanso kuchuluka kwa maselo a RPE pamagulu a 0.2% ndi 0.02%.Kutheka kwa ma cell kunakhudzidwa ndi LGSF yellowish (0.2%) ndi CB (0.2% ndi 0.02%).Mitundu iwiri ya utoto (E68 ndi BPB) sinawonetse kuopsa koyenera mu vitro.Mu kafukufukuyu mitundu inayi idadziwika kuti ili ndi zodetsa zogwira mtima, ndipo mitundu iwiri ya utotoyi ilibe mphamvu yakupha pama cell a RPE mu vitro.