Zogulitsa

Bicine ndi zwitterionic amino acid buffer, yogwira ntchito mu pH 7.6-9.0 range (pKa ya 8.26 pa 25°C).Bafa yovomerezeka ya ntchito yamankhwala am'madzi otentha otsika.Bicine imagwiritsidwa ntchito pokonza njira yokhazikika ya gawo lapansi pakutsimikiza kwa serum guanase.Kugwiritsiridwa ntchito kwa bicine mu njira yopyapyala ya ayoni yosinthira chromatography pakuwongolera mapuloteni kwasindikizidwa.Bicine yagwiritsidwa ntchito mu peptide ndi protein crystallization.Kafukufuku wa kinetic wa quaternary transition-state analogue complex of creatine kinase adagwiritsa ntchito bicine mu reaction buffer.Dongosolo lambiri lazambiri la SDS-PAGE la mapuloteni ndi ma peptides omwe amaphatikiza bicine afotokozedwa.

Makoma a alveolar amodzi omwe amaphunzira kutalika kwa saline ndi Bicine (0.2 M) amawola pang'onopang'ono mu kupsinjika kwa minofu (TTD).Tawonanso zotsatira za mayankho osiyanasiyana pa TTD iyi ndikuyang'ana zosintha zofananira mu ultrastructure.Parenchyma ya m'mapapo inapatulidwa ku makoma a alveolar amodzi (30 X 30 X 150 microns) mu saline wokhala ndi phosphate-buffered (0.15 M).Kusamutsidwa ku kusamba kwanthawi yayitali, minofuyo idamizidwa mu Bicine, saline, yankho lolimba la Hank, 0.25% Alcian blue mu saline, kapena sodium dodecyl sulfate solution, kwa nthawi zosiyanasiyana.Kudutsa muzowonjezera zoperekedwa ndi mphamvu yapamwamba yoyezedwa pakapita nthawi, minyewa yomweyi idakhazikika mu buffered glutaraldehyde/tannic acid ndikusinthidwa kuti ikhale ma electron microscopy.Makoma a alveolar amodzi omizidwa mu saline kapena Bicine adawonetsa TTD yopita patsogolo.Ma vacuoles kapena malo adawonekera mu interstitium yomwe ndi kusokonekera kwa ma cell kumapitilira ndi TTD.Kuwoneka mkati mwa 0.3 h, zosinthazo zidapita patsogolo pa 0.6 h.Mu sodium dodecyl sulfate (70 mM), komabe, munalibe TTD ndipo mwadongosolo munalibe interstitium, ndi nembanemba zapansi zokha ndi mapuloteni a fibrous otsala.Mu njira yolimba ya Hank's solution kapena 0.25% Alcian blue matrix apakati, ma cell morphology ndi kupsinjika kwa minofu zidasungidwa bwino kwa 1 h.Kafukufukuyu akuwonetsa kuti kutsika kwa matrix apakati kumachitika mu saline kapena Bicine, ndipo matrix osasunthika ndi ofunikira kuti kusungika kwa minofu.