Zogulitsa

Buffer, yogwiritsidwa ntchito pozindikira creatine-creatinase;Amagwiritsidwanso ntchito poteteza hemoglobini mu kuzizira;catholyte, yogwiritsidwa ntchito mu IEF/2-D gel electrophoresis.

Glial cell line-derived neurotrophic factor (GDNF) ndi inhibitor yamphamvu ya kumwa ethanol ndikuyambiranso, ndipo mbewa za GDNF heterozygous knockout zimawonetsa kukhudzika kwa mphotho ku ethanol ndikumamwa mowa wochulukirapo pakapita nthawi yodziletsa kuposa ma littermate awo akuthengo.Pano, tinayesa ngati ethanol imasintha mawu a GDNF m'dera la ventral tegmental (VTA; malo a GDNF) ndi / kapena nucleus accumbens (NAc; gwero lalikulu la GDNF), ndipo ngati ndi choncho, dziwani ntchito ya kukula kwa endogenous factor. pakuwongolera kumwa mowa wa ethanol.Kuwongolera mwadongosolo kwa ethanol kunachulukitsa mawu a GDNF ndi ma protein mu VTA, koma osati NAc.Kuphatikiza apo, milingo ya GDNF idakwezedwa pambuyo pa gawo lakumwa mowa wa ethanol mu makoswe omwe amadya ethanol munjira yosankha mabotolo awiri kwa sabata la 1, koma osati masabata a 7.Kutaya motsatira milungu 7 ya kumwa mopitirira muyeso kunachepetsa milingo ya GDNF, pomwe nthawi yayifupi yomwa mowa motsatizana ndi kuperewera kwa GDNF kunachepetsa mawu.Chofunika kwambiri, kugwetsa kwa GDNF mkati mwa VTA pogwiritsa ntchito adenovirus kufotokoza short hairpin RNA kunathandizira kukwera kwa mowa wa ethanol ndi makoswe a ethanol-naïve, koma osati ndi makoswe omwe amamwa mowa mopitirira muyeso.Zotsatirazi zikusonyeza kuti panthawi yoyamba kumwa mowa wa ethanol, GDNF mu VTA imawonjezeka ndipo imateteza ku chitukuko cha kumwa mowa mopitirira muyeso.Komabe, chitetezo cha kukula kwa ethanol chimasokonekera pambuyo pomwa mowa mopitilira muyeso ndikusiya, zomwe zimapangitsa kumwa mowa mopitilira muyeso.