Jeni wa beta-glucosidase (bgl3) wochokera ku Streptomyces sp.QM-B814 (American Type Culture Collection 11238) yapangidwa ndikuthandizana ndi beta-glucosidase-negative mutant ya Streptomyces lividans.Chojambula chotsegula cha 1440 nucleotides cholemba polypeptide ya 479 amino acid chinapezeka mwa kutsatizana.Mapuloteni osungidwa (Bgl3) amawonetsa kufanana kwakukulu (kupitilira 45% identity) ndi beta-glycosidases ochokera kubanja-1 glycosyl hydrolases.The enzyme cloned, yoyeretsedwa potsatira mpweya wa ammonium sulphate ndi masitepe awiri a chromatographic, ndi monomeric ndi molecular mass 52.6 kDa, monga momwe zimakhalira ndi mass spectrometry, ndi isoelectric point ya pI 4.4.Enzymeyi ikuwoneka ngati beta-glucosidase yokhala ndi gawo lalikulu la gawo lapansi, imagwira ntchito pa cellooligomers, ndipo imachita kusintha kwa transglycosylation.Mtengo wa Km wa p-nitrophenyl-beta-D-glucopyranoside ndi cellobiose ndi 0.27 mM ndi 7.9 mM, motsatana.Miyezo ya Ki ya glucose ndi delta-gluconolactone, pogwiritsa ntchito p-nitrophenyl-beta-D-glucopyranoside monga gawo lapansi, ndi 65 mM ndi 0.08 mM, motsatana.Enzyme yoyeretsedwa imakhala ndi pH optimum ya pH 6.5 ndipo kutentha kwabwino kwa ntchito ndi madigiri 50.
