Buku la β-glucosidase jini, bgl1G5, linapangidwa kuchokera ku Phialophora sp.G5 ndikufotokozedwa bwino ku Pichia pastoris.Kusanthula kwatsatanetsatane kunawonetsa kuti jini ili ndi chimango chowerengera chotseguka cha 1,431-bp cholemba puloteni ya 476 amino acid.Kutsatiridwa kwa amino acid kwa bgl1G5 kunawonetsa 85% yodziwika bwino ndi β-glucosidase yochokera ku Humicola grisea wa glycoside hydrolase banja 1. Poyerekeza ndi ma fungus ena, Bgl1G5 inawonetsa ntchito yofananira yofanana pa pH 6.0 ndi 50 ° C ndi 50 ° C. pH 5.0-9.0.Komanso, Bgl1G5 inawonetsa kutenthedwa bwino pa 50 °C (6 h theka la moyo) ndi zochitika zapamwamba kwambiri (54.9 U mg-1).Makhalidwe a K m ndi V max ku p-nitrophenyl β-D-glucopyranoside (pNPG) anali 0.33 mM ndi 103.1 μmol min–1 mg–1, motsatana.Kuyesa kwapadera kwa gawo lapansi kunawonetsa kuti Bgl1G5 inali yogwira ntchito kwambiri motsutsana ndi pNPG, yofooka pa p-nitrophenyl β-D-cellobioside (pNPC) ndi p-nitrophenyl-β-D-galactopyranoside (ONPG), ndipo inalibe ntchito pa cellobiose.
