PIPE Cas: 5625-37-6 White crystalline ufa 99% ABTS DIAMMONIUM SALT ULTRA PURE GRADE
| Nambala ya Catalog | XD90117 |
| Dzina lazogulitsa | PIPES (Piperazine-1,4-bis(2-ethanesulfonic acid) |
| CAS | 5625-37-6 |
| Molecular Formula | Chithunzi cha C8H18N2O6S2 |
| Kulemera kwa Maselo | 302.37 |
| Zambiri Zosungira | Wozungulira |
| Harmonize Tariff Code | 2933599 |
Mafotokozedwe a Zamalonda
| Zitsulo zolemera | <5ppm |
| Kutaya pa Kuyanika | <1.0% |
| Kusungunuka | Njira Yomveka, Yopanda Mtundu (5% 1N NaOH) |
| Kuyesa | 99-101% |
| Maonekedwe | White crystalline ufa |
PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] amagwiritsidwa ntchito kaŵirikaŵiri monga chotchinga pa biochemistry.Ndi ethanesulfonic acid buffer yopangidwa ndi Good et al.mu 1960s.PIPES ili ndi pKa pafupi ndi physiological pH yomwe imapangitsa kuti ikhale yothandiza pantchito zama cell.Zalembedwa kuti zichepetse kutayika kwa lipid pamene glutaraldehyde histology mu zomera ndi zinyama.
Antimicrobial peptide magainin 2 imapanga pores mu lipid nembanemba ndipo imapangitsa kuti ma membrane alowe m'ma cell.Ngakhale kuti kulowetsedwa uku ndiko chifukwa chachikulu cha ntchito yake ya bactericidal, njira yopangira pore sikudziwika bwino.Chifukwa chake tidafufuza mwatsatanetsatane momwe magiin 2 amagwirira ntchito ndi nembanemba ya lipid pogwiritsa ntchito ma giant unilamellar vesicles (GUVs).Kumangiriza kwa mainin 2 ku nembanemba ya lipid ya GUVs kunawonjezera kusintha kwapang'onopang'ono kudera la nembanemba, δ, komwe kunali kolingana ndi kuchuluka kwa magainin 2, X. Izi zikuwonetsa kuti kuchuluka kwanthawi zonse kwa magainin 2-kupangitsa awiri -kusinthika kwa dziko kuchokera ku malo osakhazikika kupita ku pore kumawonjezeka kwambiri ndi kuwonjezeka kwa δ.Kuvuta kwa nembanemba ya lipid kutsatira kulakalaka kwa GUV kudayambitsanso mapangidwe a pore a 2.Kuti awulule malo a magazine 2, kuyanjana kwa carboxyfluorescein (CF) -olembedwa m'magaziin 2 (CF-magainin 2) ndi ma GUV amodzi okhala ndi kafukufuku wa fulorosenti wosungunuka m'madzi, AF647, adafufuzidwa pogwiritsa ntchito ma microscopy osakanikirana.Popanda kukangana chifukwa cha chikhumbo, pambuyo pa kuyanjana kwa magazini 2 mphamvu ya fluorescence ya GUV rim chifukwa cha CF-magainin 2 inakula mofulumira kufika pamtengo wokhazikika, womwe unakhala wokhazikika kwa nthawi yaitali, ndi 4-32 s isanafike. kuyambika kwa kutayikira kwa AF647 kulimba kwa m'mphepete kunayamba kuwonjezeka mwachangu kupita kumtengo wina wokhazikika.Mosiyana ndi izi, pamaso pa zovutazo, palibe kuwonjezeka kwa mphamvu ya rim isanayambe kutayikira.Zotsatira izi zikuwonetsa kuti magain 2 sangathe kusuntha kuchokera kunja kupita ku monolayer wamkati mpaka atangopanga pore.Kutengera zotsatira izi, timatsimikiza kuti pore ya 2-induced pore ndi pore yotambasula ndipo kutambasula kwa mkati mwa monolayer ndiko kuyendetsa kwakukulu kwa mapangidwe a pore.
