Zogulitsa

Mayeso osavuta a malo adapangidwa, omwe amalola kuwerengera kwa microalbuminuria.Kuunikira kunachitika motsatira malangizo a ISO 15189. Mkodzo unkawoneka pamizere ya cellulose acetate ndipo udathimbirira pogwiritsa ntchito utoto wonyezimira wa mapuloteni (nigrosin, Coomassie Blue R-250, amido wakuda).Kuchuluka kwa utoto wa madontho odetsedwa kunayesedwa pogwiritsa ntchito siteshoni ya Kodak Image 450. Analytical sensitivity ya Coomassie Blue based method (18 mg/L) inali yabwino kuposa ya nigrosin (50 mg/ L) kapena amido black (100 mg/L) ) njira zozikidwa.M'kati mwa coefficient of variation (CV) ndi pakati-kuthamanga CV ya Coomassie blue assay anali, motsatira, 8.4% ndi 9.7% (50 mg/L), ndi 3% ndi 4.5% (400 mg/L).Kwa nigrosin, deta iyi inali, 8.4 ndi 9.4 (50 mg / L), ndi 3.4 ndi 6.4% (400 mg / L).Coomassie Blue adawonetsa kusankha kokonda ku albumin.Njirayi idapezeka kuti ili pakati pa 20 ndi 600 mg / L.Kulumikizana kwabwino (r2 = 0.89) kunapezedwa pakati pa Coomassie Blue based and immunonephelometric miyeso.Immuno-unreactive albumin (yokonzedwa ndi mankhwala a protease) ikhoza kuzindikirika ndi kuyesa kwa malo, komwe kumapereka mwayi wa njirayo motsutsana ndi kuyesa kwa immunochemical.Ammonium sulphate mpweya ukhoza kuonjezera kutsimikizika kwa kuyesa pochotsa zotsatira za maunyolo aulere. Njira yofotokozedwayo ndi yosavuta komanso yotsika mtengo kwambiri, yomwe imapangitsa kuti ikhale yoyenera pamapulogalamu owunika, makamaka m'maiko apadziko lonse lapansi.