Zogulitsa

Pofuna kufotokozera ndondomeko yoyamba ya mineralization ya dentini, zotsatira zolepheretsa za 1-hydroxyethylidene-1,1-bisphosphonate (HEBP) pa mineralization ya denti zinafufuzidwa.Makoswe (100 g) adalowetsedwa ndi HEBP (8 mg P / kg) kwa 7 kapena 14 d, ndipo incisors adakonzedwa kuti Ca histochemistry ndi / kapena electron microscopy.Ma incisors opangidwa ndi HEBP adawonetsa mizere yofanana ndi makwerero ya dentini ya mineralized komanso yopanda mchere kumapeto kwa apical.GBHA idawulula machitidwe apakati a Ca mu matrix osakhala ndi mineralized circumpupal dentin pomwe ma electron microscopy adawonetsa kugawa kwakukulu kwa zinthu zabwino za ma mesh ngati ma elekitironi.Matrix a dentini osapangidwa ndi mchere anali oipa kwa Ca koma anali ndi ma vesicles (MVs) ambiri odzaza ndi crystalline ndi/kapena amorphous mineral deposits.Kuchulukitsa kwa dentin wa circumpulpal kunachitika mosadalira wosanjikiza wa dentini wolemera wa MV m'zitsanzo zomwe zakhudzidwa.Zambiri zathu zimapereka umboni wa mbiri yakale wa katundu wotheka wa Ca-binding wa circumpulpal dentin matrix komanso kusapezeka kwake mu mantle dentin komwe MV-mediated mineralization imachitika.Mu chovala cha dentin, HEBP sichimasokoneza kukula kwa kristalo mu MVs koma imalepheretsa kutuluka kwake pambuyo pa kuphulika kwa membrane.Akuganiziridwa kuti matrix a circumpulpal dentin ali ndi kuthekera kopanga mineralization mosadalira MV-mediated mineralization of mantle dentin, ngakhale ma MVs amazindikira malo oyamba komanso nthawi ya mineralization ya dentini.