GSH Cas: 70-18-8 98% White crystalline ufa
| Nambala ya Catalog | XD90227 |
| Dzina lazogulitsa | Mtengo wa GSH |
| CAS | 70-18-8 |
| Molecular Formula | Chithunzi cha C10H17N3O6S |
| Kulemera kwa Maselo | 307.323 |
| Zambiri Zosungira | 2 mpaka 8 ° C |
| Harmonize Tariff Code | 29309098 |
Mafotokozedwe a Zamalonda
| Mafotokozedwe a Zamalonda | |
| Melting Point | 185 ° C |
| Gulu | Pharma kalasi |
| Zitsulo zolemera | 10ppm pa |
| Arsenic | 2 ppm pa |
| Chizindikiritso | IR: Zofanana ndi Reference Spectrum |
| Kutaya pa Kuyanika | 0.5% kuchuluka |
| Kutentha Kosungirako | + 20 ° C |
| Kulemera kwa Maselo | 612.63 |
| Kuyesa | 98.0% mphindi |
| Kuzungulira kwa Optical | -15.5 ° - -17.5 deg |
| Zotsalira pa Ignition | 0.1% kuchuluka |
| Kumveka kwa Yankho | Zomveka komanso zopanda mtundu |
| Maonekedwe | White crystalline ufa |
| Zogwirizana nazo | Chiwerengero chonse: 2.0% max, GSSG:1.5% max |
Puloteni ya ORF45 ya Kaposi's sarcoma-associated herpesvirus (KSHV) ndi puloteni ya gammaherpesvirus-yomwe imayambira nthawi yomweyo.Kafukufuku wathu wam'mbuyomu adawulula udindo wake wofunikira m'magawo oyamba komanso omaliza a matenda a KSHV.Mu phunziro ili, tinafufuza za interactome ya ORF45 pogwiritsa ntchito gulu la ma antibodies a monoclonal.Kuphatikiza pa mapuloteni omwe adadziwika kale a extracellular regulated kinase (ERK) ndi p90 ribosomal S6 kinase (RSK), tidapeza mapuloteni ena angapo opangidwanso, kuphatikiza odziwika a ~ 38 kDa ndi ~ 130 kDa.Mass spectrometry idawulula kuti puloteni ya 38-kDa ndi ma virus ORF33 ndipo mapuloteni a 130-kDa ndi ma USP7 (ubiquitin-specific protease 7).Tinapanga mapu a ORF33-binding domain ku carboxyl-terminal 19 amino acid (aa) yosungidwa kwambiri ya ORF45 ndi USP7-binding domain ku ndondomeko yogwirizana yomwe inanenedwa m'chigawo chapakati cha ORF45.Pogwiritsa ntchito immunofluorescence staining, tinawona colocalization ya ORF45 ndi ORF33 kapena USP7 zonse pansi pa ma ion opatsirana komanso m'maselo omwe ali ndi kachilombo ka KSHV.Komanso, tinawona kusuntha kwa ORF45 kwa gawo la ORF33/USP7 kuchokera pamphuno kupita ku cytoplasm.Tinapeza kuti ORF45 inachititsa kuwonjezeka kwa mapuloteni a ORF33 omwe anathetsedwa ngati ORF33- kapena USP7-binding domain mu ORF45 inachotsedwa.Kuphatikiza apo, kuchotsedwa kwa carboxyl terminus yosungidwa ya ORF45 mu genome ya KSHV kunachepetsa kwambiri kuchuluka kwa mapuloteni a ORF33 m'maselo omwe ali ndi kachilombo ka KSHV ndikuthetsa kupanga ma virioni a ana.Zonse pamodzi, zotsatira zathu sizimangosonyeza zigawo zatsopano za ORF45 interactome, komanso zimasonyeza kuti kugwirizana pakati pa mapuloteniwa n'kofunika kwambiri kuti KSHV lytic replication.Kaposi's sarcoma-associated herpesvirus (KSHV) ndizomwe zimayambitsa makhansa angapo a anthu.KSHV ORF45 ndi mapuloteni ambiri omwe amafunikira KSHV lytic replication, koma njira zenizeni zomwe ORF45 imagwirira ntchito zake zovuta sizidziwika bwino.Kafukufuku wathu wam'mbuyomu adawonetsa kuti ma ORF45 pr otein onse m'maselo amapezeka m'magulu olemera kwambiri.Chifukwa chake tidafuna kuwonetsa kuyanjana kwa ORF45 kuti timvetsetse ntchito zake panthawi yobwerezabwereza.Pogwiritsa ntchito gulu la ma antibodies a monoclonal, tinafufuza za ORF45 interactome m'maselo omwe ali ndi kachilombo ka KSHV.Tinazindikira mabwenzi awiri atsopano omangirira a ORF45: mapuloteni a viral ORF33 ndi ma cell ubiquitin-specific protease 7 (USP7).Tikuwonetsanso kuti kuyanjana pakati pa ORF45 ndi ORF33 ndikofunikira pakupanga bwino kwa tinthu tating'onoting'ono ta KSHV, kutanthauza kuti kusokoneza komwe kumakhudzana ndi kuyanjanaku kungayimire njira yatsopano yoletsa kubwereza kwa KSHV.
