Zogulitsa

Hepatotoxicity ya omwe akufuna kugwiritsa ntchito mankhwala osokoneza bongo ndi chimodzi mwazinthu zomwe zimadetsa nkhawa kwambiri pakuwunika kwamankhwala pakupezeka koyambirira kwa mankhwala.Kuzindikira kupsinjika kwa hepatic oxidative kumatha kukhala chizindikiro choyambirira cha hepatotoxicity ndikupindulitsa kusankha mankhwala.Gulu la glutathione (GSH) ndi glutathione disulfide (GSSG) pair, monga imodzi mwazinthu zazikulu zoyendetsera ma redox omwe amawongolera maanja, amagwira ntchito yofunika kwambiri poteteza maselo ku nkhawa ya okosijeni yomwe imayamba chifukwa cha kusalinganika pakati pa prooxidants ndi antioxidants.Kutsimikiza kwa kuchuluka kwa ma GSSG / GSH ndi kuchuluka kwa GSH ndi GSSG kwagwiritsidwa ntchito kuwonetsa kupsinjika kwa okosijeni m'maselo ndi minofu.Mu kafukufukuyu, tidayesa kuthekera kogwiritsa ntchito ma biliary GSSG/GSH ratios ngati biomarker kuwonetsa kupsinjika kwa hepatic oxidative komanso poizoni wamankhwala.Mankhwala anayi omwe amadziwika kuti amasintha ma GSH ndi GSSG adayesedwa mu phunziroli.Diquat (diquat dibromide monohydrate) ndi acetaminophen adaperekedwa kwa makoswe.Paraquat ndi tert-butyl hydroperoxide ankaperekedwa kwa mbewa kuti apangitse kusintha kwa biliary GSH ndi GSSG.Ma biliary GSH ndi GSSG adayesedwa pogwiritsa ntchito ma curve owongolera okonzedwa ndi bile opangira kuti awerengere zotsatira za bile matrix pakuwunika kwa LC-MS ndikupewa kusokoneza kwa GSH ndi GSSG.Ndi zitsanzo zinayi (mu makoswe ndi mbewa) za kusintha kwa mankhwala osokoneza bongo mu kinetics ya biliary GSSG / GSH ratios, phunziroli linasonyeza kuthekera kopanga ndondomeko yoyankhira yokhudzana ndi biliary GSSG / GSH ratios yolosera kupsinjika kwa hepatic oxidative.