Zogulitsa

Pathogenic molecular pathways mu matenda a cirrhotic chiwindi monga hepatitis C virus (HCV), autoimmune hepatitis (AIH) ndi primary biliary cirrhosis (PBC) sadziwika bwino.Ma jini ofotokozedwa mosiyanasiyana nthawi zambiri amakhala ofunikira pakuyambitsa matenda.Suppression subtractive hybridization (SSH) ndi njira yofalikira ya ma genome yomwe imathandizira zolemba za mRNA mosiyanasiyana.Tidafuna kuti tiwone momwe ma jini amasiyanitsira mu cirrhosis pogwiritsa ntchito SSH kuphatikiza ndi kuchuluka kwa nthawi yeniyeni reverse transcriptase polymerase chain reaction (RT-PCR).Ma transcriptomes a chiwindi mu HCV cirrhosis, AIH cirrhosis, PBC, ndi minyewa yachiwindi yosadziwika idawunikidwa ndi SSH.Ma DNA (cDNA) ophatikizana omwe adatsatira adawunikidwanso kuti awonetsere kusiyana ndi kusakanizidwa kwa madontho kenako ndikutsatiridwa.Mafotokozedwe a jini osankhidwa adatsimikiziridwa ndi nthawi yeniyeni RT-PCR.Kutsatira SSH, ma clones 694 adawunikidwanso kuti awonetsere ma jini osiyanasiyana, omwe 145 adatsatiridwa ndipo adapezeka kuti amachokera kumitundu yosiyanasiyana ya 89.Ma clones asanu ndi awiri anali ofanana okha ndi ma encoding tag (EST) omwe alibe ntchito yodziwika.Mafotokozedwe apamwamba a majini anayi adatsimikiziridwa ndi nthawi yeniyeni RT-PCR: transmembrane 4 superfamily membala 3 (tetraspanin CO-029) mu mitundu yonse ya cirrhosis, hedgehog interacting protein (HIP) mu AIH cirrhosis ndi chitinase 3-like-1 (HC gp-39 kapena ykl-40) ndi arginine-glutamic acid kubwereza (RERE) mu HCV cirrhosis.RERE gene polymorphisms ndi mitundu yosiyanasiyana ya splice idawonedwa m'magulu onse omwe adawunikidwa.Tetraspanin CO-029 up-regulation idayikidwa m'maselo a bile ductular.Pomaliza, zidziwitso zatsopano zamafotokozedwe amitundu yosiyanasiyana mu cirrhosis yamunthu zidapangidwa pogwiritsa ntchito SSH ngati chida choyambirira chodziwira.Makamaka, maphunziro owonjezera a jini ya RERE ndi zinthu zake mu HCV yokhudzana ndi matenda a chiwindi ndizoyenera.