Zogulitsa

Amagwiritsidwa ntchito popanga buffer ya CAPS, buffer ya zwitterionic yothandiza mu pH 7.9-11.1.CAPS buffer imagwiritsidwa ntchito kwambiri pakuyesa kwa Western ndi immunoblotting komanso kutsatizana kwa mapuloteni ndi kuzindikira.Amagwiritsidwa ntchito potumiza mapuloteni a electro ku PVDF (sc-3723) kapena nembanemba ya nitrocellulose (sc-3718, sc-3724).Kuchuluka kwa pH kwa chotchinga ichi kumapangitsa kukhala kothandiza kusamutsa mapuloteni okhala ndi pI> 8.5.ndi reactivity yochepa ndi michere kapena mapuloteni, mchere zochepa zotsatira.

Mu capillary zone electrophoresis mphamvu ya electrophoretic ya ion imachepa pamene ndende ya maziko a electrolyte yankho ikuwonjezeka.Izi zimachitika chifukwa cha kusintha kwa electrophoretic mobility ya ion (muep) komanso kusintha kwa mphamvu ya ukonde yomwe ikukhudza, yomwe ndi mphamvu yamagetsi yamagetsi (Eeff).Mayendedwe a electrophoretic a ion amasinthidwa kudzera mukusintha kwamphamvu kwamphamvu kwa electrolyte solution ndikusintha kukula kwa ayoni.Eeff imasinthidwa makamaka ndi kusintha kwa kukula kwa mtengo wa asymmetry effect ndi electrophoretic effect, zomwe zimalepheretsa ma ion kuyenda.Mu phunziro ili, njira ya zizindikiro zitatu idagwiritsidwa ntchito pofufuza zotsatira za ndende ya electrolyte (0.02-0.08M 3--[cyclohexylamino] -1-propanesulfonic acid ndi counter ion (Li, Na, K, ndi Cs) pa Eeff. Zinapezeka kuti kuchuluka kwa ma electrolyte akumbuyo kumakhudza kwambiri Eeff komanso kuti Eeff imayandikira E pomwe kuchuluka kwa ma electrolyte akuyandikira ziro. , Eeff inachepera.