Zogulitsa

Zotsatira za acidosis, chizindikiro cha biochemical cha chotupacho, pakukula kwa khansa ndi metastasis ndizovuta.Zothandizira ndi zotsutsana ndi zotupa za acidosis zanenedwa ndipo acidic microenvironment yagwiritsidwa ntchito popereka mankhwala enieni, ojambula zithunzi, ndi majini opangidwa kukhala zotupa.Mu kafukufukuyu tikufufuza za kufalikira ndi kumamatira kwa maselo a melanoma a B16F10 omwe amapangidwa kuti awonetsere pH-sensing G protein-coupled receptor GPR4.Pogwiritsa ntchito ma cell attachment assay tinapeza kuti GPR4 overexpression inachedwetsa kufalikira kwa maselo ndikusintha kufalikira kwa malo a dynamic focal adhesion complex, monga kumasulira kwa phosphorylated focal adhesion kinase (FAK) ndi paxillin, pa acidic pH.Kuthekera kwa ma G-protein ndi njira zolozera kunsi kwa mitsinje zomwe zimayambitsa zotsatirazi zidafufuzidwanso.Pogwiritsa ntchito Rho inhibitor CT04 (C3 transferase), Rho-associated kinase (ROCK) inhibitors Y27632 ndi thiazovivin, myosin light chain kinase (MLCK) inhibitor staurosporine kapena G12/13 inhibitory construct, kufalikira kwa maselo kunabwezeretsedwa pamene inhibitor kutsegulira kwa njira za Gq ndi Gs kunali ndi zotsatira zochepa kapena palibe.Zotsatira zathu zonse zikuwonetsa kuti kudzera mu njira ya G12/13/Rho GPR4 imathandizira kukhazikika kwamphamvu ndikuchepetsa kufalikira kwa ma cell ndi kugwedezeka kwa membrane.