Zogulitsa

Ma zwitterionic butanesulfonic acid atsopano omwe amakulitsa kuchuluka kwa alkaline m'mabanja anayi a Good buffers: kuwunika kuti agwiritsidwe ntchito muzinthu zachilengedwe.

Ma buffer anayi atsopano a zwitterionic butanesulfonic acid omwe amalumikizana mwadongosolo ndi mabanja anayi a Good buffers adawunikidwa kuti agwiritsidwe ntchito muzinthu zachilengedwe.Ma buffers awa, okhala ndi ma pKa values ​​kuchokera ku 7.6 mpaka 10.7, adafanizidwa ndi ma buffer ena osiyanasiyana ochokera kubanja lomwelo komanso ndi zotchingira zosagwirizana kuti adziwe momwe amakhudzira ntchito ya enzyme komanso kukula kwa tizilombo.Ntchito ya ma enzymes anayi omwe ali ndi pH yabwino kwambiri mumtundu wa alkaline adayesedwa: beta-galactosidase, esterase, phosphodiesterase ndi alkaline phosphatase.Nthawi zambiri, ma buffers onse Abwino, kuphatikiza mabafa atsopano a butanesulfonic acid, adapereka ntchito yabwino;komabe, panali kusintha kwa magwiridwe antchito a michere ina yokhala ndi mabafa ena.Tris, glycine, ndi phosphate buffers nthawi zambiri amawonetsa kusiyanasiyana kwa zochitika poyerekeza ndi banja la Good buffers.beta-Galactosidase, makamaka, adawonetsa zochitika zazikulu zokhala ndi zotchingira Zabwino kuposa ndi phosphate kapena Tris buffers.Mofananamo, kukula kwa mitundu isanu ndi iwiri ya mabakiteriya kunali kofanana, kupatulapo zochepa, kwa Banja Labwino la ma buffers omwe ali ndi Tris nthawi zambiri amalepheretsa kukula.Kuchulukitsidwa kwa alkaline phosphatase yolumikizidwa ndi ma antibodies ndi chida chofunikira kwambiri pakugwiritsa ntchito ma cell a biology.Ma buffer angapo Abwino adapereka zidziwitso zabwino poyerekeza ndi Tris pa pH 9.5 pozindikira mapuloteni pogwiritsa ntchito ma antibodies a alkaline phosphatase-conjugated.