Zogulitsa

The histamine H4 receptor (HRH4), yomwe idapezeka zaka 13 zokha zapitazo, imawonedwa ngati chandamale chamankhwala chodalirika cha ziwengo, kutupa, kusokonezeka kwa autoimmune ndi khansa, monga zikuwonekera ndi kuchuluka kwachulukidwe kwa zofalitsa zasayansi ndi kugwiritsa ntchito patent.Ngakhale kukhalapo kwa HRH4 kwatsimikiziridwa mu testis, malo ake enieni kapena ntchito yake sizinakhazikitsidwe.Apa, tidafuna kudziwa kukhudzidwa kwa HRH4 pakuwongolera magwiridwe antchito a cell a Leydig.Tidayesa koyamba mawonekedwe ake m'maselo a chotupa a MA-10 Leydig ndikuwunika zotsatira za ma agonists awiri a HRH4 pa steroidogenesis ndi kuchuluka.Tidapeza kuti HRH4 imawonetsedwa bwino m'maselo a MA-10, ndikuti kuyambitsa kwake kumabweretsa kuletsa kwa LH/chorionic gonadotropin-induced cAMP kupanga ndi kuwonetsa kwa protein ya Star.Kuphatikiza apo, tidawona kuchepa kwa kuchuluka kwa maselo pambuyo pa chithandizo cha agonist cha 24-h HRH4.Kenako tidazindikira malo a HRH4 muyeso wanthawi zonse, ndipo tidazindikira kuti HRH4 imateteza makoswe m'maselo a Leydig azaka za 7-240, pomwe makoswe amasiku 21 adawonetsanso mawu a HRH4 mumagametes aamuna.Pomaliza, tidawunika momwe HRH4 imayankhira pakuchulukira kwa kholo labwinobwino komanso mbewa ya Leydig cell cell, ndipo onse adawonetsa kuti amatha kutengeka ndi anti-proliferative effect ya HRH4 agonists.Popeza kufunikira kwa histamine (2-(1H-imidazol-4-yl) ethanamine) mu physiology yaumunthu (patho) physiology, kuyesayesa kopitilira kumayang'aniridwa pakuwunikira zomwe zikubwera za HRH4 ndi ma ligand ake.Kafukufukuyu akuwonetsa masamba atsopano a mawu a HRH4, ndipo akuyenera kuganiziridwa popanga ma HRH4 agonists osankhidwa pazifukwa zochiritsira.