Zogulitsa

CTP:phosphocholine cytidylyltransferase α (CCTα) ndi puloteni ya nyukiliya yomwe imayambitsa sitepe yochepetsera mlingo mu CDP-choline pathway for phosphatidylcholine (PC) synthesis.Lipid activation ya CCTα imapangitsa kuti isamutsidwe ku envelopu ya nyukiliya ndikukula kwa netiweki ya intranuclear netiweki yotchedwa nucleoplasmic reticulum (NR) ndi makina ophatikizira nembanemba.Miyendo ya nyukiliya imafunikanso kuti pakhale bata ndi kufalikira kwa NR, koma ngati mawonekedwe apaderawa, kapena lamina ya nyukiliya yonse, ikufunika kuti kaphatikizidwe ka PC sikadziwika.Kuti muwone ubalewu, lamina ya nyukiliya inathetsedwa ndi RNAi kapena kusokonezedwa ndi mawu a Hutchinson-Gilford progeria syndrome (HGPS) mutant lamin A (progerin), ndipo zotsatira za CCTα ndi choline metabolism zinafufuzidwa.SiRNA-mediated kutonthola kwa lamin A/C kapena lamin B1 m'maselo a CHO kuti achepetse NR sikunakhudze kaphatikizidwe ka PC, pomwe kugogoda kawiri sikunatsekereza njirayo.Kutsimikizira t gawo lake laling'ono mu kaphatikizidwe ka PC, 10% yokha ya choline / ethanolamine phosphotransferase yokhazikika yomwe idapezeka mu NR.M'maselo a CHO, CCTα inali nucleoplasmic ndi co-localized ndi GFP-progerin mu nyukiliya folds ndi invaginations;komabe, HGPS fibroblasts inawonetsa kugawidwa kwachilendo kwa CCTα mu cytoplasm ndi envelopu ya nyukiliya yomwe inatsagana ndi kuchepetsedwa kwa 2 mu PC synthesis.Ngakhale kuti kusinthidwa kwake kumasinthidwa, kuyesa kwa choline-labeling kunasonyeza kuti ntchito ya CCT sinakhudzidwe, ndipo kulepheretsa kaphatikizidwe ka PC kunayambika kuchepetsedwa kwa ntchito ya hemicholinium-sensitive choline transporter.Timatsimikiza kuti CCTα ndi lamin zimagwirizana kuti apange NR, koma mawonekedwe onse a envelopu ya nyukiliya ali ndi zotsatira zochepa pa ntchito ya CCT ndi kaphatikizidwe ka PC.