Zogulitsa

tRNA ndi gulu losinthidwa kwambiri la mitundu ya RNA, ndipo zosinthidwa zimapezeka mu tRNAs kuchokera ku zamoyo zonse zomwe zawunikidwa.Ngakhale kuti ali ndi mitundu yosiyanasiyana yamankhwala komanso kupezeka kwawo m'matRNA osiyanasiyana, omwe amapezeka m'malo osiyanasiyana mu tRNA, njira za biosynthetic za zosintha zambiri za tRNA zikuphatikiza masitepe a methylation.Zomwe zapezedwa posachedwapa zavumbulutsa zovuta zomwe sizinachitikepo m'machitidwe osinthika a tRNA, kuwongolera ndi magwiridwe antchito, kuwonetsa kuti nucleoside iliyonse yosinthidwa mu tRNA ikhoza kukhala ndi ntchito yakeyake.Komabe, muzomera, chidziwitso chathu pa ntchito ya kusintha kwa tRNA payekha komanso momwe imayendetsedwa ndi yochepa kwambiri.Mu mawonekedwe a majini opangidwa kuti azindikire zinthu zomwe zimayang'anira kukana matenda ndi kuyambitsa chitetezo ku Arabidopsis, tazindikira SUPPRESSOR WA CSB3 9 (SCS9).Zotsatira zathu zimawulula SCS9 encodes a tRNA methyltransferase yomwe imayimira 2'-O-ribose methylation ya mitundu yosankhidwa ya tRNA mu antico don loop.Zosintha za SCS9-mediated tRNA zimachulukirachulukira munthawi ya matenda a bakiteriya a Pseudomonas syringae DC3000, komanso kusowa kwa kusintha kwa tRNA, monga momwe zimawonera mu scs9 mutants, zimasokoneza kwambiri chitetezo cham'mera polimbana ndi kachilombo komweko popanda kukhudza chizindikiro cha salicylic acid (SA) njira yomwe imayambitsa chitetezo cha mthupi.Zotsatira zathu zimathandizira chitsanzo chomwe chimapereka kufunikira kwa kuwongolera kwa kusintha kwina kwa tRNA kuti akhazikitse chitetezo chamthupi ku Arabidopsis, motero amakulitsa mndandanda wamagulu amagulu ofunikira kuti athe kuyankha moyenera matenda.