Zogulitsa

4-Methylumbelliferyl β-D-glucopyranoside yakhala ikugwiritsidwa ntchito ngati gawo lapansi: · mu glucosylceramidase β enzyme assay mu tizigawo tomwe timapangidwa ndi lysosome kuchokera ku primary hippocampal neurons · mu β-glucosidase assay pa yeast fermentation · to assay glusebrosedase-1GB ntchito mu macrophage cell line (RAW)

4-Methylumbelliferyl β-D-glucopyranoside ndi gawo lopangira enzymatic la glycosidase.Amagwiritsidwa ntchito ngati gawo lapansi la β-glucosidase kuchokera ku enterococci.

Tikunena kuti opereka glycoside osavuta amatha kusintha kwambiri momwe glycosyltransferase-catalyzed reaction, amasintha mapangidwe a shuga a NDP kuchokera ku endothermic kupita ku njira yotentha.Kuwonetsa kugwiritsa ntchito kusinthasintha kwa thermodynamic uku, tikuwunikira glycosyltransferase-catalyzed synthesis ya 22 nucleotides shuga kuchokera kwa opereka shuga onunkhira bwino, komanso kupanga kofananira kwa ma nucleotides a shuga monga mphamvu yoyendetsera glycosyltransferase-catalyzed reactions for yaing'ono-molecule glycoversification.Othandizira onunkhira osavuta awa adathandiziranso kuyesa kwamitundu yonse kwa glycosyltransfer, komwe kumagwiritsidwa ntchito popezeka ndi mankhwala, uinjiniya wamapuloteni ndi kufufuza kwina kodalira shuga wa nucleotide.Kafukufukuyu akutsutsa mwachindunji lingaliro lachidziwitso lakuti NDP-shuga ndi opereka shuga 'owonjezera mphamvu' pamene achotsedwa pa chikhalidwe chawo chachilengedwe.