Zogulitsa

Protein phosphorylation imayang'anira ntchito zosiyanasiyana zama cell ndipo imathandizira kwambiri pakukula koyambirira kwa zomera.Kuti tithandizire ndikukulitsa kafukufuku wam'mbuyomu wa mapuloteni a phosphorylation mu mbande za Arabidopsis tidagwiritsa ntchito njira ina yomwe imaphatikiza kutulutsa mapuloteni pansi pamikhalidwe yopanda denaturing ndi immobilized metal-ion affinity chromatography (IMAC) kulemeretsa kwa ma phosphoprotein osakhazikika muzotulutsa za Rubisco, ndikutsatiridwa ndi chizindikiritso. pogwiritsa ntchito gel electrophoresis ya mbali ziwiri (2-DE) ndi madzi chromatography-tandem mass spectrometry (LC-MS/MS).Mu-gel trypsin digestion ndi kusanthula kwa mawanga osankhidwa a gel osankhidwa adazindikira ma peptide 144 a phosphorylated ndi zotsalira, zomwe 18 phosphopeptides ndi 8 phosphosites zokha zidapezeka m'madatabase a PhosPhAt 4.0 ndi P3DB Arabidopsis thaliana phosphorylation.Oposa theka la ma phosphoproteins odziwika a 82 anali nawo mu metabolism ya carbohydrate, photosynthesis / kupuma kapena oxidative stress response mechani sms.Kulemera kwa ma phosphoprotein osakhazikika asanafike 2-DE ndi LC-MS/MS akuwoneka kuti amathandizira kuzindikira kwa phosphorylated threonine ndi zotsalira za tyrosine poyerekeza ndi njira zomwe zimagwiritsira ntchito kuchulukitsa kwa peptide, kutanthauza kuti njira ziwirizi ndizogwirizana pang'ono pokhudzana ndi kufalikira kwa malo a phosphorylation.Poyerekeza zotsatira za mbande zazing'ono ndi zomwe zidapezedwa kale masamba okhwima a Arabidopsis adazindikira mapuloteni asanu omwe ali ndi phosphorylated m'mafupawa, kuwonetsa kuthekera kwa njirayi pofufuza mphamvu ya mapuloteni a phosphorylation pakukula kwa mbewu.