Zogulitsa

Madzi sungunuka reagent kwa enzymatic photometric kutsimikiza wa hydrogen peroxide.Ma reagents atsopano a Trinder ndizomwe zimasungunuka m'madzi za aniline zomwe zimagwiritsidwa ntchito kwambiri poyesa mayeso ndi mayeso a biochemical.Pali maubwino angapo kuposa ochiritsira chromogenic reagents pa colorimetric kutsimikiza kwa hydrogen peroxide ntchito.Ma reagents atsopano a Trinder ndi okhazikika mokwanira kuti agwiritsidwe ntchito mu njira zonse zothetsera komanso kuyesa njira zowunikira mapaipi.Pamaso pa hydrogen peroxide ndi peroxidase, buku la Trinder's reagent lidawonetsedwa kuti likuchita ndi 4-aminoantipyrine (4-AA) kapena 3-methylbenzothiazole sulfonehydrazone (MBTH) panthawi ya oxidative coupling reaction.Amapanga utoto wokhazikika wa violet kapena buluu.Kutsekemera kwa molar kwa utoto wophatikizidwa ndi MBTH kunali 1.5-2 nthawi yayitali kuposa ya utoto wophatikizidwa ndi 4-AA;komabe, yankho la 4-AA linali lokhazikika kuposa yankho la MBTH.Gawo lapansi limapangidwa ndi oxidase kuti lipange hydrogen peroxide.Kuchuluka kwa hydrogen peroxide kumafanana ndi gawo lapansi.Choncho, kuchuluka kwa gawo lapansi kungadziwike ndi kukula kwa mtundu wa oxidative coupling reaction.Glucose, mowa, acyl-CoA ndi cholesterol zitha kugwiritsidwa ntchito kuzindikira magawo omwe aphatikizidwa ndi buku la Trinder's reagent ndi 4-AA.Pali ma reagents 10 atsopano a Trinder omwe akupezeka.Pakati pa ma reagents atsopano a Trinder, TOOS ndi omwe amagwiritsidwa ntchito kwambiri.Komabe, pagawo linalake, kuyesa magulu osiyanasiyana a ma reagents a Trinder ndikofunikira kuti mupange njira yodziwira bwino.

Madzi sungunuka reagent kwa enzymatic photometric kutsimikiza wa hydrogen peroxide.Ma reagents atsopano a Trinder ndizomwe zimasungunuka m'madzi za aniline zomwe zimagwiritsidwa ntchito kwambiri poyesa mayeso ndi mayeso a biochemical.Pali maubwino angapo kuposa ochiritsira chromogenic reagents pa colorimetric kutsimikiza kwa hydrogen peroxide ntchito.Ma reagents atsopano a Trinder ndi okhazikika mokwanira kuti agwiritsidwe ntchito mu njira zonse zothetsera komanso kuyesa njira zowunikira mapaipi.Pamaso pa hydrogen peroxide ndi peroxidase, buku la Trinder's reagent lidawonetsedwa kuti likuchita ndi 4-aminoantipyrine (4-AA) kapena 3-methylbenzothiazole sulfonehydrazone (MBTH) panthawi ya oxidative coupling reaction.Amapanga utoto wokhazikika wa violet kapena buluu.Kutsekemera kwa molar kwa utoto wophatikizidwa ndi MBTH kunali 1.5-2 nthawi yayitali kuposa ya utoto wophatikizidwa ndi 4-AA;komabe, yankho la 4-AA linali lokhazikika kuposa yankho la MBTH.Gawo lapansi limapangidwa ndi oxidase kuti lipange hydrogen peroxide.Kuchuluka kwa hydrogen peroxide kumafanana ndi gawo lapansi.Choncho, kuchuluka kwa gawo lapansi kungadziwike ndi kukula kwa mtundu wa oxidative coupling reaction.Glucose, mowa, acyl-CoA ndi cholesterol zitha kugwiritsidwa ntchito kuzindikira magawo omwe aphatikizidwa ndi buku la Trinder's reagent ndi 4-AA.Pali ma reagents 10 atsopano a Trinder omwe akupezeka.Pakati pa ma reagents atsopano a Trinder, TOOS ndi omwe amagwiritsidwa ntchito kwambiri.Komabe, pagawo linalake, kuyesa magulu osiyanasiyana a ma reagents a Trinder ndikofunikira kuti mupange njira yodziwira bwino.