Zogulitsa

Kusiyanitsa kwa orbital fibroblasts kukhala adipocyte okhwima komanso kudzikundikira kwa minofu ya adipose kwawonetsedwa pakupita patsogolo kwa Graves 'orbitopathy (GO).Autophagy imakhudzidwa ndi adipogenesis, koma zochepa zomwe zimadziwika za gawo la autophagy pakuyambitsa ndi kupita patsogolo kwa GO.Cholinga cha phunziroli ndi kufufuza ntchito ya autophagy mu matenda a GO.Orbital adipose / connective tissue explants kuchokera kwa odwala omwe ali ndi GO ndi kuchokera ku maphunziro abwino, komanso osungulumwa a orbital fibroblasts, adafufuzidwa.Adipogenesis ananyengedwa pogwiritsa ntchito kusiyanitsa sing'anga ndi kapena popanda hydrogen peroxide, ndipo autophagy inasinthidwa pogwiritsa ntchito bafilomycin A1 ndi Atg5-targeted short hairpin RNA (shRNA).Ma autophagosomes adadziwika ndi ma electron microscopy.Mafotokozedwe a majini okhudzana ndi autophagy ndi zolembera zokhudzana ndi adipogenesis adawunikidwa ndi nthawi yeniyeni yosinthira-polymerase chain reaction ndi/kapena kusanthula kwa Western blot.Kuchulukana kwa madontho a Lipid kunayesedwa ndi Mafuta Ofiira Oyipitsitsa. Ma vacuoles a autophagic anali ochuluka kwambiri m'maselo a GO kusiyana ndi omwe sanali GO (p <0.05).Mafotokozedwe a majini okhudzana ndi autophagy anali apamwamba kwambiri mumagulu a GO ndi ma cell kuposa anzawo omwe sali GO, motsatana.Interleukin-1β yowonjezera LC3-II, p62, ndi Atg7 mapuloteni mu GO maselo.Kudzikundikira kwa autophagosome kunawonetsedwa pa tsiku la 4 la adipogenesis ndikuchepera pa tsiku la 10, komanso kupanga madontho a lipid.Mafotokozedwe a mapuloteni a LC3 ndi p62 anawonjezeka mkati mwa maola 48 akusiyana ndipo anachepa pang'onopang'ono kuyambira tsiku la 4 mpaka 10. Chithandizo cha Bafilomycin A1 ndi Atg5 knockdown ndi shRNA inhibited lipid droplet kudzikundikira ndi kuponderezedwa kwa zizindikiro za adipogenic. Autophagy inawonjezeka mu GO minofu ndi maselo poyerekeza ndi GO minyewa ndi ma cell omwe si a GO, kutanthauza kuti autophagy imakhala ndi gawo mu GO pathogenesis.Kuwongolera kwa Autophagy kumatha kukhala chandamale chachipatala cha GO.