PMSF Cas: 329-98-6 98.0% woyera crystalline ufa Phenylmethanesulfonyl fluoride (PMSF)
| Nambala ya Catalog | XD90250 |
| Dzina lazogulitsa | Phenylmethanesulfonyl fluoride (PMSF) |
| CAS | 329-98-6 |
| Molecular Formula | C7H7FO2S |
| Kulemera kwa Maselo | 174.1927 |
| Zambiri Zosungira | Wozungulira |
| Harmonize Tariff Code | 29049900 |
Mafotokozedwe a Zamalonda
| Kuyesa | ≥98.0% HPLC |
| Maonekedwe | woyera crystalline ufa |
PMSF ndi serine/cysteine protease inhibitor yosasinthika yomwe imagwiritsidwa ntchito popanga ma cell lysates.
Maphunziro a in vitro: PMSF (2 mM) inaletsa carbachol-stimulated inositol phosphate kudzikundikira ndi 15% -19% yokha pamaso pa Li+.Kuletsa kwa phosphoinositide kutembenuka kwa PMSF kumachitika chifukwa cha sitepe imodzi kapena zingapo kutsatira kuwonongeka kwa phosphoinositides [1].PMSF imalepheretsa acylation ya inositol zotsalira za GPI intermediates m'magazi a T. brucei.PMSF imalepheretsa mapangidwe a glycolipid C koma osati mafuta okonzanso mu vitro.PMSF imalepheretsa kuwonjezera kwa GPI acylation ndi ethanolamine phosphatase mu procyclic trypanosomes, koma osati m'maselo a Hela [2].
Maphunziro a mu vivo: PMSF (0.1 mL / 10 g b.wt, ip) inapanga antinociception, monga momwe ziwonetsedwera ndi kuwonjezeka kwa mlingo wa% MPE mu tail-flick latency assessment, koma inalephera kutulutsa zoletsa zomveka zomvera mlingo.Makoswe omwe adalandira jekeseni wa intraperitoneal wa PMSF adawonetsa zotsatira za cannabinoid kuphatikiza antinociception, hypothermia ndi kusasunthika ndi ED50 values za 86, 224 ndi 206 mg / kg, motsatana.PMSF (30 mg/kg) pretreatment imawonjezera zotsatira za anandamide pamayankho a mchira (antinociception), ntchito ya locomotor ndi kuyenda ndi 5-fold, 10-fold and 8-fold, motsatana [3].
Kuyesa kwa nyama: Makoswe aamuna a ICR olemera 18 mpaka 25 g adagwiritsidwa ntchito poyesa.PMSF inasungunuka mu mafuta a sesame ndipo imayendetsedwa intraperitoneally mu voliyumu ya 0.1 mL/10 g b.wt.Nthawi zonse perekani PMSF mphindi 10 musanayambe jekeseni wa anandamide kapena galimoto.Mbewa zinazolowera chipinda choyesera usiku wonse popanda kusokoneza chakudya kapena madzi.Kutsatira mtsempha wa anandamide kapena kayendetsedwe ka galimoto, chiweto chilichonse chinayesedwa motere: Mphindi 5 kwa mayankho a mchira-flick latency (antinociceptive) ndi 5 kwa mphindi 15 pazochitika zokhazokha (motor);kapena mphindi 5 pa kutentha kwapakati (rectal) ndi ring Immobilization (catalepsy) kwa mphindi 5 mpaka 10.
