Zogulitsa

Ngakhale kuti fluoride imadziwika kuti imapangitsa kuti mafupa apangidwe, zomwe zimapangidwira sizimamveka bwino.Kafukufuku waposachedwapa wakhudza njira ya Wnt/β-catenin ngati njira yaikulu yowonetsera mafupa.Kafukufuku wathu wam'mbuyomu adawonetsa gawo lotheka la njira yovomerezeka ya Wnt pakupanga mafupa a makoswe osawoneka bwino a fluoride, koma makinawo sakudziwika bwino.Kafukufuku wamakono adatsimikizira kukhudzidwa kwa Wnt / β-catenin chizindikiro mu fluoride-induced osteoblastic differentiation.Pogwiritsa ntchito makoswe osteoblasts, tidawonetsa kuti fluoride imalimbikitsa kwambiri kuchuluka kwa osteoblasts ndi mawu a alkaline phosphate (ALP) komanso mafotokozedwe a mRNA a zolembera zosiyanitsa mafupa, kuphatikiza mtundu wa I collagen (COL1A1), ALP ndi osteonectin.Tinapezanso kuti fluoride induced phosphorylations pa serine 473 ya Akt ndi serine 9 ya glycogen synthase kinase-3β (GSK3β), yomwe inachititsa kuti GSK-3β inhibition ndipo kenako kusonkhanitsa nyukiliya kwa β-catenin, monga momwe Western blot ndi immunofluoresce zikuwonekera. .Kuphatikiza apo, fluoride idapangitsanso mawu a Wnt-targeted gene runt-related transcription factor 2 (Runx2).Chofunika kwambiri, zotsatira zabwino za fluoride pa ntchito ya ALP ndi mafotokozedwe a mRNA a COL1A1, ALP, osteonection ndi Runx2 anathetsedwa ndi DKK-1, blocker ya Wnt/β-catenin receptor.Kuphatikizidwa pamodzi, zomwe zapezazi zikusonyeza kuti fluoride imalimbikitsa kusiyana kwa osteoblastic kudzera mu Akt- ndi GSK-3β-odalira kutsegula kwa njira yowonetsera ya Wnt / β-catenin mu primary rat osteoblasts.Zomwe tapeza zimapereka zidziwitso zatsopano zamachitidwe a fluoride mu osteoblastogenesis.