Zogulitsa

LecA (PA-IL) ndi lectin ya cytotoxic ndi zomatira zomwe zimapangidwa ndi Pseudomonas aeruginosa zomwe zimamanga galactosides ya hydrophobic ndi kutsimikizika kwakukulu komanso kuyanjana.Pogwiritsa ntchito kaphatikizidwe ka lecA-egfp ndi kusanthula kwa immunoblot kwa biofilm extracellular matrix, tikuwonetsa kuti lecA imawonetsedwa m'maselo opangidwa ndi biofilm.Mu kuyesa kwa static biofilm pa polystyrene ndi chitsulo chosapanga dzimbiri, kuya kwa biofilm ndi kuphimba pamwamba kunachepetsedwa ndi masinthidwe a lecA ndi kulimbikitsidwa mu mtundu wa LecA-ochulukitsa kwambiri PAO-P47.Kuphimba kwa biofilm pamwamba pa zovuta za makolo, PAO-P47 koma osati lecA mutant pa makuponi achitsulo kunaletsedwanso ndi kukula pamaso pa isopropyl-beta-D-thiogalactoside (IPTG) kapena p-nitrophenyl-alpha-D-galactoside ( NPG).Kuphatikiza apo, ma biofilms okhwima akuthengo opangidwa popanda ma hydrophobic galactosides amatha kumwazikana powonjezera IPTG.Mosiyana ndi zimenezi, kuwonjezera kwa p-nitrophenyl-alpha-L-fucose (NPF) yomwe ili ndi chiyanjano chachikulu cha P. aeruginosa LecB (PA-IIL) lecti n sichinakhudze kupanga biofilm kapena kubalalitsidwa.Kukula kwa planktonic kwa P. aeruginosa PAO1 sikunakhudzidwe ndi kupezeka kwa IPTG, NPG kapena NPF, komanso kupsinjikako sikunathe kugwiritsa ntchito mashugawa ngati magwero a kaboni, kutanthauza kuti zotsatira zomwe zawonedwa pakupanga mapangidwe a biofilm zidachitika chifukwa choletsa mpikisano wa LecA-ligand. kumanga.Zotsatira zofananazo zinapezedwanso za biofilms zomwe zimakula pansi pa mikhalidwe yothamanga pa makuponi achitsulo, kutanthauza kuti LecA imathandizira ku P. aeruginosa biofilm zomangamanga pansi pa zochitika zosiyanasiyana zachilengedwe.

Alpha-L-fucosidase (EC 3.2.1.51) yokhoza kumasula zotsalira za t-fucosyl kuchokera kumbali ya xyloglucan oligosaccharides yapezeka m'masamba a zomera za Arabidopsis.Komanso, alpha-L-fucosidase yokhala ndi gawo limodzi lofananira idayeretsedwa kuchokera ku masamba a kabichi (Brassica oleracea) kuti apange gulu limodzi pa SDS-PAGE.Njira ziwiri za peptide zidapezedwa kuchokera ku gulu la mapuloteniwa, ndipo zidagwiritsidwa ntchito kuzindikira ma jini a Arabidopsis a alpha-fucosidase omwe tikufuna kuyitcha AtFXG1.Kuphatikiza apo, jini ya Arabidopsis yokhala ndi homology yodziwika bwino ya alpha-L-fucosidase yapezekanso, ndipo tidafuna kuitcha kuti AtFUC1.Onse AtFXG1 ndi ATFUC1 adawonetsedwa mosiyanasiyana m'maselo a Pichia pastoris ndi zochitika za alpha-L-fucosidase zobisidwa kwa sing'anga ya chikhalidwe.Alpha-L-fucosidase yolembedwa ndi AtFXG1 inali yogwira ntchito motsutsana ndi oligosaccharides kuchokera ku xyloglucan XXFG komanso motsutsana ndi 2'-fucosyl-lactitol koma osati motsutsana ndi p-nitrophenyl-alpha-L-fucopyranoside.Komabe, AtFUC1 yofotokozedwa momveka bwino inali yogwira ntchito motsutsana ndi 2'-fucosyl-lactitol.Chifukwa chake, choyambiriracho chiyenera kukhala chogwirizana ndi xyloglucan metabolism.