Zogulitsa

Acridine ester (NSP-SA-NHS) Cas199293-83-9 ndi mankhwala ogwirizana nawo ndi opindulitsa kwambiri zilembo za chemiluminescent zomwe kukhazikika, ntchito ndi kukhudzika kwake kumaposa ma radioisotopes ena.Acridine esters amatha kuchitapo kanthu ndi mapuloteni okhala ndi magulu oyambira amino.Pansi pa zofunikira, NHS imalowetsedwa ngati gulu lochoka, ndipo mapuloteni amapanga mgwirizano wokhazikika wa amide ndi acridine ester.Zomwezo zitatha, mchere wochuluka wa acridinium unachotsedwa kudzera muzakudya za desalting.

Mapuloteni otchedwa acridine safuna enzymatic catalysis kuti atulutse kuwala pamaso pa hydrogen peroxide yamchere.Mfundo yeniyeni yotulutsa kuwala ndi yakuti mu njira ya alkaline ya hydrogen peroxide, acridine ester amawukiridwa ndi ayoni a hydrogen peroxide kuti apange dioxyethane yosasunthika ndi zovuta, zomwe zimawonongekanso kukhala CO2 ndi acridone wokondwa pakompyuta.Pamene acridone ibwerera ku nthaka, imatulutsa ma photons okhala ndi kutalika kwa kutalika kwa 430 nm.Njira ya luminescence iyi ndi yaifupi kwambiri (njira yonseyi imatenga masekondi osachepera 2), ndipo ndondomeko yowonongeka iyenera kuwonjezera photometer yamkati ndi photon detector;Kuphatikiza apo, mankhwalawa amathanso kugwiritsa ntchito chowerengera chamitundu yambiri chokhala ndi ma autosampler osonkhanitsira deta ya luminescence.Mapuloteni, ma peptides, ma antibodies, ndi ma nucleic acid onse amatha kulembedwa ndi mankhwalawa.Acridine esters amatulutsa kuwala mofulumira pansi pa chisangalalo cha alkaline hydrogen peroxide, kotero kuti mankhwala olembedwa amatha kuzindikirika posonkhanitsa photons.

Izi zimagwiritsidwa ntchito makamaka: chemiluminescence ndi immunoassay, kusanthula kwa receptor, nucleic acid ndi peptide kuzindikira ndi kafukufuku wina.