Zogulitsa

Njira yolimba ya immunoassay yodziwira progesterone mu plasma yaumunthu ikufotokozedwa, yomwe imagwiritsa ntchito chemiluminescence ngati mapeto.progesterone-isoluminol conjugate imakhala ngati chemiluminescent marker.Kagawo kakang'ono ka IgG ka antiserum kupita ku progesterone-11 alpha-hemisuccinate bovine serum albumin imalowetsedwa m'makoma a machubu oyesa a Lumacuvettes P polystyrene.Pambuyo pomangiriza, ma cuvettes amatsukidwa.Kutulutsa kowala kwa ma conjugate omangidwa pa oxidation ndi H2O2-microperoxidase system pa pH 13 kunatsika ndikuwonjezeka kwaulere kwa progesterone munjira yolumikizana ndi chipika pamtunda wa 15-1000 pg/chubu.Chemiluminescence immunoassay ndi yofanana ndi radioimmunoassay pokhudzana ndi kukhudzidwa, kutsimikizika, kulondola komanso kulondola.Kuyesa kumapereka ubwino wa liwiro komanso kuphweka kwa makina.Kuonjezera apo, njirayi simaphatikizapo kugwiritsa ntchito ma radioisotopes kapena sitepe ya centrifugation.