Zogulitsa

Mu njira ya kinetic angiotensin-converting enzyme (ACE), chosungira chothandiza komanso choyenera ndi 80 mmol/L borate buffer pa pH 8.2 (37 degrees C).Gawo losakhalitsa limadziwika ndi momwe zimachitikira, ndipo kulowetsedwa kwa 5-min wa gawo lapansi ndi madzi a m'magazi kumanenedwa musanayambe kuyeza kwa kinetic.Gawo laling'ono, N- [3- (2-furyl) acryloyl] -L-phenylalanylglycylglycine (FAPGG), ndende imakwezedwa pa 1.0 mmol/L ndipo kutalika kwa muyeso kumakhala pa 345 nm kuti zitsimikizire mzere woyezera.Njira yomwe ikufunsidwa imagwiritsa ntchito chiŵerengero cha 1: 9 plasma-to-reagent volume.Mizere yoyeserera imafikira pafupifupi 170 U/L, kuyimira 25% gawo lapansi la hydrolysis.Kuyamwa kwa FAPGG kumatsimikiziridwa poyesa kusiyana kwa kuyamwa pakati pa 1.0 mmol/L FAPGG ndi mayankho azinthu.Kukhulupirika kwa wavelength kumawunikiridwa pozindikira kuchuluka koyembekezeka kwa yankho la FAPGG, ndipo kupatuka kwa 1.0-nm kuchokera ku 345 nm kumasintha kuyamwa ndi 15.5%.Kulondola kwa mayeso a ACE pafupifupi 60 ndi 100 U/L ndi 3.5% ndi 2.4% mkati mwa batch ndi 2.9% ndi 2.6% pakati pa batch, motsatana.Nthawi yofotokozera (2.5th mpaka 97.5th percentiles) ndi 41-139 U/L, ndipo palibe kusiyana pakati pa zikhalidwe za amuna ndi akazi.