γ-L-Glutamyl-3-carboxy-4-nitroanilide, mchere wa monoammonium Cas: 63699-78-5 Kuwala kwachikasu microcrystalline ufa
| Nambala ya Catalog | XD90183 |
| Dzina lazogulitsa | γ-L-Glutamyl-3-carboxy-4-nitroanilide, mchere wa monoammonium |
| CAS | 63699-78-5 |
| Molecular Formula | C12H12N3O7-.H4N+ |
| Kulemera kwa Maselo | 328.27 |
| Zambiri Zosungira | Wozungulira |
Mafotokozedwe a Zamalonda
| Maonekedwe | Kuwala chikasu microcrystalline ufa |
| Asay | 99% |
| Malo osungunuka | 187ºC ± 2.0ºC |
| Malo otentha | 718.3 ° C pa 760 mmHg |
| pophulikira | 368.5 °C |
| Kusungunuka | H2O: 100 mg/mL, yowoneka bwino, yobiriwira yachikasu |
The kinetics of human serum gamma-glutamyltransferase (EC 2.3.2.2) anafufuzidwa, pogwiritsa ntchito glycylglycine monga gamma-glutamyl kulandira gawo lapansi ndi gamma-glutamyl-4-nitroanilide ndi carboxy yochokera ku carboxy, gamma-glutamyl-3-carboxy-4 -nitroanilide, monga gawo lapansi lopereka.Kuchitika nthawi imodzi kwa gamma-glutamyltransfer ndi autotransfer kudakhazikitsidwa ndi kutsika kwapa chromatography.Magawo owirikiza kawiri-owirikiza kawiri amatsimikizira kuti makina a enzyme ndi osafunikira (ping-pong bi-bi).Kuletsa kwa wopereka aliyense sikunapezeke, ndipo kuletsa kwa glycylglycine kumawonedwa kokha pazambiri zomwe zimaposa zomwe zimakhudzidwa ndichipatala.Zosintha za Kinetic zomwe zimapezedwa ndi kusanthula kosasinthika kwa data yoyambira kuthamanga zidagwiritsidwa ntchito kudziwa kuchuluka kwa reagent gawo lapansi pakuyesa kwa enzyme iyi.Kuyeza kwa 4 mmol ya gamma-glutamyl-3-carboxy-4-nitroanilide ndi 100 mmol wa glycylglycine pa lita imodzi kunawonetsa ntchito zofanana ndi zomwe zimagwiritsidwa ntchito poyesa 4 mmol ya gamma-glutamyl-4-nitroanilide ndi 40 mmol glycylglycine pa lita imodzi.Kuchulukitsitsa kwa carboxy donor ndi glycylglycine ndi "mtengo wokwanira" ndipo sikubweretsa zovuta pamachitidwe akagwiritsidwa ntchito.
