Zogulitsa

Carbonic anhydrase activators: kafukufuku woyamba woyambitsa wachinsinsi wa isoform VI wokhala ndi ma amino acid ndi ma amines.

The secretory isozyme ya human carbonic anhydrase (hCA, EC 4.2.1.1), hCA VI, yapangidwa, kufotokozedwa, ndi kuyeretsedwa mu dongosolo lofotokozera mabakiteriya.Ma kinetic parameters a CO(2) hydration reaction adatsimikizira hCA VI kukhala ndi ak(mphaka) wa 3.4 x 10(5)s(-1) ndi k(mphaka)/K(M) wa 4.9 x 10(7)M (-1)s(-1) (pa pH 7.5 ndi 20 digiri C).hCA VI ali ndi chidwi chothandizira ntchito zokhudza thupi anachita, chimodzimodzi dongosolo la ukulu monga ubiquitous isoform CA I kapena transmembrane, chotupa-zogwirizana isozyme CA IX.Mndandanda wa ma amino acid ndi ma amines adawonetsedwa kuti azichita ngati oyambitsa CA VI, okhala ndi magwiridwe antchito osiyanasiyana.l-His, l-Trp, ndi dopamine adawonetsa zofooka za CA VI activating zotsatira (K (A) m'kati mwa 21-42 microM), pamene d-His, d-Phe, l-DOPA, l-Trp, serotonin , ndipo ena pyridyl-alkylamines anali activator bwino, ndi K(A)s mu osiyanasiyana 13-19 microM.Othandizira kwambiri a CA VI anali l-Phe, d-DOPA, l-Tyr, 4-amino-l-Phe, ndi histamine, okhala ndi K(A)s mumitundu ya 1.23-9.31 microM.Izi zoyambitsa zonse zimakulitsa k(mphaka), osakhudza K(M), kutenga nawo gawo pamlingo wodziwikiratu kayendetsedwe kazinthu, kusintha kwa pulotoni pakati pa enzyme yogwira ntchito ndi chilengedwe.