Zogulitsa

Infrared spectroscopy yakhala ikugwiritsidwa ntchito popanga kugwirizana kwa mapuloteni a gawo lapansi: kusintha kosinthika kwa sarcoplasmic reticulum Ca(2+)-ATPase pa nucleotide binding ndi ATPase phosphorylation adayang'aniridwa pogwiritsa ntchito gawo lapansi la ATP ndi ATP analogues (2'-deoxy-ATP, 3). '-deoxy-ATP, ndi inosine 5'-triphosphate), zomwe zinasinthidwa pamagulu apadera a gawo lapansi.Kusintha kwa 2'-OH, 3'-OH, ndi gulu la amino la adenine kumachepetsa kuchuluka kwa kusintha kwa ATPase komwe kumapangidwira, ndi zotsatira zamphamvu zomwe zimawonedwa kwa awiri omaliza.Izi zikuwonetsa kukhudzidwa kwamapangidwe a nucleotide-ATPase zovuta pakulumikizana kwamunthu pakati pa nucleotide ndi ATPase.Magulu onse omwe amaphunzira ndi ofunikira kumangirira ndi kuyanjana kwa gulu la ligand lomwe linapatsidwa ndi ATPase zimadalira kuyanjana kwa magulu ena a ligand.Phosphorylation ya ATPase idawonedwa pa ITP ndi 2'-deoxy-ATP, koma osati 3'-deoxy-ATP.Palibe kulumikizana kwachindunji pakati pa kuchuluka kwa kusintha kosinthika pakumangiriza kwa ma nucleotide ndi kuchuluka kwa phosphorylation kuwonetsa kuti kuchuluka kwa kusintha kosinthika kopangidwa ndi ATP sikuli kovomerezeka kwa phosphorylation.Monga momwe tawonera pa nucleotide-ATPase complex, conformation ya phosphorylated ATPase yapakati ya E1PCa (2) ya phosphorylated imatengeranso nucleotide, kusonyeza kuti mayiko a ATPase ali ndi mawonekedwe ochepa kwambiri kuposa momwe ankayembekezera.