Zogulitsa

Adenosine ndi endogenous regulator of vascular tone.Ntchitoyi ya adenosine imathetsedwa ndi kutengeka kwake ndi kagayidwe kake ndi maselo a microvascular endothelial cell (MVEC).Chonyamula chachikulu chomwe chikukhudzidwa ndi ENT1 (equilibrative nucleoside transporter subtype 1).MVEC imasonyezanso nucleobase transporter (ENBT1) yomwe imakhudzidwa ndi kutuluka kwa ma cell adenosine metabolites monga hypoxanthine.Kusintha kwa machitidwe onsewa kungakhudze bioactivity ya adenosine ndi metabolism yake, kuphatikizapo mapangidwe a oxygen free radicals.MVEC otalikirana ndi chigoba minofu ya ENT1(+/+) ndi ENT1(-/-) mbewa anali pansi ndi okosijeni kupsyinjika anachititsa ndi yoyerekeza ischemia/reperfusion kapena menadione.Zochita za ENT1 ndi ENBT1 zidawunikidwa kutengera ziro-trans influx kinetics of substrates zolembedwa ma radio.Panali kuchepa kwa mlingo wa ENBT1-mediated hypoxanthine uptake ndi ENT1(+/+) MVEC wothandizidwa ndi menadione kapena atakumana ndi zinthu zomwe zimatsanzira isc hemia/reperfusion.M'zochitika zonsezi, superoxide dismutase mimetic MnTMPyP inachepetsa kutayika kwa ntchito ya ENBT1, kutanthauza ma radicals a superoxide poyankha.Mosiyana ndi zimenezi, mbewa za MVEC zodzipatula ku ENT1(-/-) mbewa sizinawonetse kuchepa kwa ntchito za ENBT1 pochiza ndi menadione kapena kuyerekezera ischemia/reperfusion, koma anali ndi ntchito yapamwamba kwambiri ya catalase poyerekeza ndi ENT1(+/+) MVEC.Deta iyi imasonyeza kuti ntchito ya ENBT1 yachepetsedwa mu MVEC chifukwa cha kuwonjezeka kwa superoxide radical yomwe imagwirizanitsidwa ndi ischemia / reperfusion kuvulala.MVEC yotalikirana ndi ENT1(-/-) mbewa siziwonetsa kuchepa uku kwa ENBT1, mwina chifukwa cha kuchuluka kwa ntchito za catalase.