Zogulitsa

1.Njira za mbiri ya Metabolite ndi zida zofunika zoyezera madziwa a metabolite muzinthu zachilengedwe.Ngakhale njira zambiri zofotokozera za metabolite zimawonetsa kulimba kwapakatikati kapena zimadalira miyeso yochepa yamkati yomwe imayimira ma metabolites onse, chofunikira kwambiri pakufotokozera kuchuluka kwa dziwe la metabolite m'maselo achilengedwe ndi zamadzimadzi ndikutsimikiza kokhazikika.Timapereka lipoti pano za njira yowunikira kwambiri komanso yowoneka bwino ya gasi chromatography/tandem mass spectrometry (GC/MS/MS) yolunjika pa mbiri ya metabolite yomwe imathandizira kuchulukitsidwa kokwanira kwa ma metabolite onse omwe apezeka.Njirayi idakhazikitsidwa ndi methyl chloroformate derivatization ndi kuchuluka kwa ma spiking samples okhala ndi miyezo ya metabolite padera yotengedwa ndi deuterated derivatization reagents.Ma ionization amtundu wa elekitironi amalowetsedwa m'malo ndi ionization yamankhwala abwino popeza omalizawo mpaka pamlingo wokulirapo amasunga ma ion a molekyulu ndi zidutswa zina zolemera kwambiri zama cell.Izi zidapangitsa kuti zikhale zosavuta kusankha masinthidwe apadera a MS/MS pakati pa ma metabolites ambiri ophatikizika.Pakadali pano, njira yatsopano ya GC/MS/MS ili ndi 67 metabolites wamba omwe ambiri amakhala m'magulu a amino ndi nonamino organic acid.Tikuwonetsa kugwiritsidwa ntchito kwa njirayi pamikodzo ndi zitsanzo za seramu.Njirayi ndikusintha kwakukulu kwa njira zomwe zilipo za kuchuluka kwa GC/MS metabolite ma amino acid ndi nonamino organic acid.

2.24S-Hydroxycholesterol (24OHC) ndi 27-hydroxycholesterol (27OHC) ndi ma oxysterol awiri ofanana mosiyanasiyana - omwe kale anali opangidwa muubongo ndipo omalizawo amapangidwa pang'ono muubongo kuposa ziwalo zina zambiri.ZOYENERA KUYESA: Kuwonongeka kwa mitsempha ndi / kapena kuchepa kwa mitsempha kumayambitsa kuwonjezereka kwa 24OHC kuchokera ku ubongo kupita ku cerebrospinal fluid (CSF), pamene chotchinga chamagazi-muubongo chimayambitsa kuwonjezereka kwa 27OHC kuchokera kumayendedwe kupita ku CSF.Isotope dilution-mass spectrometry inagwiritsidwa ntchito poyesa ma oxysterols awiri mu CSF ndi plasma kuchokera kwa odwala oposa 250 omwe ali ndi matenda osiyanasiyana a mitsempha ndi geriatric. Miyezo ya CSF ya ma oxysterol awiriwa inakhudzidwa kwambiri ndi matenda osiyanasiyana kusiyana ndi ma plasma.Odwala ndi matenda yogwira demyelinating anali kuchuluka milingo 24OHC mu CSF ndi mkulu 24OHC/27OHC chiŵerengero.Odwala ndi meningitis ambiri anali ndi milingo yambiri ya ma steroids okhala ndi chiŵerengero chochepa cha 24OHC/27OHC.Odwala omwe ali ndi matenda a Alzheimer's anali ndi milingo yowonjezereka ya 24OHC mu CSF ndikuwonjezeka pang'ono kwa 27OHC.Odwala omwe ali ndi multiple sclerosis anali ndi chizolowezi chokhala ndi 24OHC pa nthawi yogwira ntchito ndi chiwerengero chachikulu cha 24OHC / 27OHC. Kuyeza kwa ma oxysterols awiri mu CSF ndi plasma kungapangitse kwambiri njira zomwe zilipo kale zowunikira matenda a ubongo.