Zogulitsa

Ma cell a mesenchymal stem cell (MSCs) adanenedwa kukhala gwero lowoneka bwino lopanga ma cell a β cell osinthika.Mzere wa cell wa embryonic mesenchymal progenitor cell C3H10T1/2 wazindikirika ngati chitsanzo cha ma MSC, chifukwa cha kuthekera kwake kosiyanitsa mibadwo yambiri.Cholinga cha kafukufukuyu chinali kufufuza ngati maselo a C3H/10T1/2 ali ndi kuthekera kosiyana ndi ma cell omwe amapanga insulin (IPCs).Apa, tidafufuza ndikuyerekeza kusiyanitsa kwa makoswe a MSC ndi ma C3H10T1/2 kukhala ma IPC.Maselo atatha kusiyanitsa kwa IPC, mawu osiyanitsa amazindikiridwa ndi immunocytochemistry, reverse transcript-polymerase chain reaction (RT-PCR), kuchuluka kwa nthawi yeniyeni RT-PCR (qRT-PCR) ndi kutseka kwa Western.Kutulutsa kwa insulin kumawunikidwa ndi enzyme-linked immunosorbent assay (ELISA).Kuphatikiza apo, maselo osiyanitsidwawa adasinthidwa kukhala mbewa za matenda ashuga a streptozotocin ndipo ntchito zawo zachilengedwe zidayesedwa mu vivo.Kafukufukuyu akuwonetsa njira ya 2 yopangira ma IPC kuchokera ku maselo a C3H10T1/2.Pansi pa zochitika zapadera za masiku 7-8, maselo a C3H10T1 / 2 amapanga matupi a spheroid atatu-dimensional (SBs) ndi kutulutsa insulini, pamene mbadwo wa IPCs wochokera ku makoswe a MSC unkafunika nthawi yaitali (kuposa masabata a 2).Kuphatikiza apo, ma IPC awa opangidwa kuchokera ku ma cell a C3H10T1/2 adabayidwa mbewa za matenda ashuga ndikuwongolera shuga woyambira, kulemera kwa thupi ndikuwonetsa kuyezetsa koyenera kwa glucose.Kafukufuku wapano adapereka chitsanzo chosavuta komanso chodalirika cha in vitro kuti apitirize kufufuza momwe IPC imasiyanitsira ma MSC ndi ma cell m'malo mwa matenda a shuga.