Zogulitsa

Membala watsopano wa banja la AP2/ERF transcription factor, GmERF3, anali atalekanitsidwa ndi soya.Kusanthula kwatsatanetsatane kunawonetsa kuti GmERF3 ili ndi AP2/ERF ya 58 amino acid ndi madera awiri a putative nuclear localization sign (NLS).Anali a gulu la IV mapuloteni mu ERF (ethylene response factor) subfamily monga momwe amachitira ndi N-terminal motif yosungidwa [MCGGAI (I/L)].Kufotokozera kwa GmERF3 kudayambitsidwa ndi mankhwala okhala ndi mchere wambiri, chilala, abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), ethylene (ET), ndi kachilombo ka soya mosaic (SMV), pomwe panalibe GmERF3 yofunikira. mRNA kudzikundikira pansi ozizira nkhawa mankhwala.GmERF3 imatha kumangirira ku bokosi la GCC ndi chinthu cha DRE/CRT, ndipo idalunjika ku nyukiliyasi ikawonetsedwa kwakanthawi m'maselo a anyezi a epidermal.Puloteni ya GmERF3 idasakanikirana ndi domeni yomanga ya GAL4 DNA kuti ayambitse kulembedwa kwa majini atolankhani mu yisiti.Mawonekedwe a Ectopic a jini ya GmERF3 muzomera zafodya wamtundu wina adalimbikitsa kufotokozera kwa mitundu ina ya PR komanso kulimbikira kukana matenda a Ralstonia solanacearum, Alternaria alternata, ndi fodya wa fodya mosaic virus (TMV), ndikupangitsa kulekerera kupsinjika kwa mchere wambiri komanso kuchepa kwa madzi m'thupi.Kuphatikiza apo, kuchulukitsidwa kwa GmERF3 mufodya wa transgenic kunapangitsa kuti pakhale ma proline aulere komanso ma carbohydrate osungunuka poyerekeza ndi zomera zakutchire pansi pa chilala.Zotsatira zonse zikuwonetsa kuti GmERF3 ngati AP2/ERF transcript factor itha kugwira ntchito ziwiri poyankha kupsinjika kwa biotic ndi abiotic muzomera.