D-Luciferin Cas: 2591-17-5 99% Yoyera mpaka ufa wachikasu nbsp BEETLE LUCIFERIN
| Nambala ya Catalog | XD90248 |
| Dzina lazogulitsa | D-Luciferin |
| CAS | 2591-17-5 |
| Molecular Formula | Chithunzi cha C11H8N2O3S2 |
| Kulemera kwa Maselo | 280.323 |
| Zambiri Zosungira | -15 mpaka -20 ° C |
| Harmonize Tariff Code | 29342080 |
Mafotokozedwe a Zamalonda
| M'madzi | Max.2.0% |
| Chiphuphu | Max 2.0 NTU |
| Kuzungulira kwapadera kwa kuwala | -36 mpaka -32 |
| Maonekedwe | Ufa woyera mpaka wachikasu |
| Purity HPLC | Zochepera 99% |
| Molar extinction coefficient | mphindi 17900 L/(mol cm) |
Chiyambi: D-luciferin ndi gawo lapansi la adenosine triphosphate (ATP)-yodalira bioluminescence reaction.Mfundo ya bioluminescence ndi yakuti luciferin imatulutsidwa ndi luciferase pamaso pa ATP ndi mpweya.The chemical reaction formula ndi motere: ATP+D-Luciferin+O2→Oxyluciferin+AMP+PPi+O2+Kuwala.
Njira yochitira: Njira yogwiritsira ntchito D-luciferin ndikuti pansi pa ATP ndi luciferase, luciferin (gawo lapansi) likhoza kukhala oxidized kuti litulutse kuwala.Pamene luciferin ikupitirira, chiwerengero cha photons chopangidwa chimagwirizana bwino ndi kuchuluka kwa luciferase.
Ntchito: D-luciferin ndi gawo lapansi la adenosine triphosphate (ATP) -yodalira bioluminescence reactions.Bioluminescence reaction ya luciferin/luciferase nthawi zambiri imagwiritsidwa ntchito pozindikira ATP, metabolites yomwe ingasinthidwe kukhala ATP (monga AMP, ADP, cAMP), ndi michere yomwe imatha kupanga ATP (monga creatine kinase, etc.), bioluminescence reaction ingagwiritsidwe ntchito.Imagwira ntchito pozindikira mitundu yosiyanasiyana yazachilengedwe.
Ntchito Zachilengedwe: D-Luciferin (Firefly luciferin) ndi gawo lapansi lodziwika bwino la bioluminescence pamaso pa ATP kuti ligwiritsidwe ntchito mu kujambula kwa luciferase-based bioluminescence imaging ndi ma cell-based high-throughput screening.
Maphunziro a in vitro: D-luciferin imakhudzidwa ndi luciferase, ATP ndi mpweya kuti utulutse kuwala, komwe kumadziwika ndi filimu yojambula zithunzi kuti muwone ma antibodies omangidwa ndi alkaline phosphatase.
Maphunziro a mu vivo: kugwiritsa ntchito magawo a D-luciferin ndi ziphaniphani luciferase kumateteza kuyanjana kwa chitetezo chamthupi ndi chotupa mu mtundu wa mbewa wosagwira ntchito ndi khansa ya ovarian, popeza mapulogalamu a bioluminescence amakhudzidwa kwambiri ndi kukula kwa chotupa kuposa malangizo owonjezera kulemera kwa thupi.
