Zogulitsa

Ma brucellae ndi omwe amayambitsa matenda a brucellosis, zoonosis yofalikira padziko lonse lapansi.Mabakiteriyawa ndi tizilombo toyambitsa matenda ndipo motero amatha kusintha kagayidwe kawo kumalo owonjezera ndi ozungulira omwe amakumana nawo panthawi ya matenda.Komabe, mbali iyi ya biology ya Brucella imamveka bwino, ndipo zakudya zomwe zimapezeka mu niche ya intracellular sizidziwika.Apa, tidafufuza njira zapakati za C metabolism yomwe Brucella abortus amagwiritsa ntchito pochotsa putative fructose-1,6-bisphosphatase (fbp ndi glpX), phosphoenolpyruvate carboxykinase (pckA), pyruvate phosphate dikinase (ppdK), ndi malic enzyme (mae). majini.Mu gluconeogenic koma osati muzofalitsa zolemera, kukula kwa ΔppdK ndi Δmae mutants kunali kofooka kwambiri ndipo kukula kwawiri Δfbp-ΔglpX mutant kunachepetsedwa.Mu macrophages, okhawo ΔppdK ndi Δmae mutants adawonetsa kuchepa kwachulukidwe, ndipo maphunziro ndi ΔppdK mutant adatsimikizira kuti adafika pa niche yobwerezabwereza.Momwemonso, ma ΔppdK ndi Δmae osinthika okha omwe adasinthidwa ndi mbewa, omwe kale adatsukidwa ndi sabata 10 ndipo omalizawo amapitilira masabata a 12.Tidafufuzanso kuzungulira kwa glyoxylate.Ngakhale ntchito yolimbikitsa ya aceA (isocitrate lyase) idakulitsidwa m'njira yolemera, kusokoneza kwa aceA sikunachite mu vitro kapena kuchulutsa mu macrophages kapena mbewa spleens.Zotsatira zimasonyeza kuti B. abortus imakula mozungulira pogwiritsa ntchito mphamvu zochepa za 6-C (ndi 5-C) shuga zomwe zimalipidwa ndi glutamate ndipo mwina ma amino acid ena omwe amalowa mu Krebs popanda ntchito yovuta ya glyoxylate shunt.