Zogulitsa

Chiyambi: BSA ndi imodzi mwamapuloteni omwe amagwiritsidwa ntchito kwambiri m'ma laboratories a biochemical, ndipo kufunikira kwake kutha kunyalanyazidwa poyesera chifukwa ndiyofala kwambiri komanso yamba.Bovine serum albumin (BSA), yomwe imadziwikanso kuti gawo lachisanu, ndi globulin mu seramu ya bovine yomwe ili ndi zotsalira za amino acid 583, yokhala ndi molekyulu yolemera 66.430kDa ndi isoelectric point ya 4.7.BSA ili ndi machitidwe osiyanasiyana pakuyesa kwa biochemical, monga blocking agent ku Western blots.

Kugwiritsa Ntchito: Bovine Serum Albumin (BSA), yomwe imadziwikanso kuti gawo lachisanu, ndi globulin mu seramu ya bovine, yomwe ili ndi zotsalira za amino acid 607, ndipo imakhala ndi ntchito zambiri pakuyesa kwachilengedwe.Bovine seramu albumin amagwiritsidwa ntchito ngati stabilizer mu njira yosungirako ndi njira yothetsera ma enzymes oletsa kapena ma enzymes osinthidwa kuti akhazikitse ntchito ya enzyme ndikuletsa kuwonongeka kwa enzyme komanso kutsatsa kosadziwika.

Ntchito: BSA imagwiritsidwa ntchito ngati stabilizer mu njira yosungiramo njira yosungiramo komanso njira yothetsera ma enzymes oletsa kapena ma enzymes osinthidwa, chifukwa ma enzymes ena amakhala osakhazikika kapena amakhala ndi zochitika zochepa pazambiri zotsika.Pambuyo powonjezera BSA, ikhoza kukhala ndi gawo la "chitetezo" kapena "chonyamulira", ndipo ntchito ya michere yambiri imatha kusintha kwambiri pambuyo powonjezera BSA.Ma enzymes omwe safuna kuwonjezera kwa BSA nthawi zambiri samakhudzidwa ndi kuwonjezera kwa BSA.Kwa DNA yambiri ya gawo lapansi, BSCA imatha kupangitsa kuti chimbudzi chikhale chokwanira, ndipo chimatha kukwaniritsa kudula mobwerezabwereza.Pa 37 ° C, pamene chimbudzi chimaposa 1 h, BSA ikhoza kupangitsa kuti puloteni ikhale yolimba, chifukwa muzomwe zimapangidwira popanda BSA, ma enzymes ambiri oletsa amatha kupulumuka pa 37 ° C kwa 10 ~ 20min kapena nthawi yochepa..Mosiyana ndi izi, BSA imatha kumanga ma ayoni achitsulo ndi mankhwala ena mu buffer kapena gawo lapansi la DNA lomwe limaletsa ntchito yoletsa ma endonucleases.

Ntchito: Standard grade bovine serum albumin (BSA, StandardGrade), yomwe imatha kukwaniritsa zofunikira zambiri zoyesera, monga immunoblocking agent, cell cell (tizilombo toyambitsa matenda ndi tizilombo toyambitsa matenda, etc.) zakudya zamtundu ndi zigawo za chikhalidwe, kukhazikika kwa mapuloteni reagents ndi mapuloteni quantification miyezo.Diagnostic grade bovine serum albumin (BSA, DiagnosticGrade) imatha kukwaniritsa zosowa zambiri zoyesera, monga immunoblocking agent, protein/enzyme stabilizer, diluent, carrier and protein standard.Kuphatikiza apo, itha kugwiritsidwanso ntchito poyesa ma immunoassays, chikhalidwe cha ma cell ndi kuyesa kosakanizidwa komwe kumafunikira chidwi chachikulu.