Zogulitsa

Mitundu ya uropathogenic ya Escherichia coli (UPEC) ndizomwe zimayambitsa matenda a bacteremic mkodzo.Kupulumuka m'magazi kumagwirizanitsidwa ndi njira zosiyanasiyana zomwe zimathandiza kukana kuphana kwa seramu-mediated.Ngakhale kuti phenotypic heterogeneity ya mabakiteriya yasonyezedwa kuti imakhudza kulekerera kwa maantibayotiki, mwayi woti umapangitsa kuti maselo asaphedwe ndi chitetezo cha mthupi sichinayesedwe moyesera.Pakafukufuku wapano tidafuna kudziwa ngati kusiyanasiyana kwa zikhalidwe za mabakiteriya ndizogwirizana ndi kutsata kwa bakiteriya ndi seramu complement system.Tidayang'anira magawo a cell mu mtundu wa UPEC CFT073 wokhala ndi mapuloteni a fluorescent reporter.Maselo okhazikika amalowetsedwa mu seramu yamunthu yogwira kapena yosatenthedwa ngati palibe maantibayotiki osiyanasiyana (ampicillin, norfloxacin, ndi amikacin), komanso kugawikana kwa ma cell ndikumangirira kwa mapuloteni a C3 kunayesedwa ndi ma flow cytometry ndi ma immunofluorescence microscopy.Heterogeneity mu kuwirikiza kawiri kwa maselo a CFT073 mu seramu kunapangitsa kuti magawo atatu a phenotypically osiyana asiyanitsidwe, onsewo akudziwika ndi gawo la C3 la dongosolo lothandizira.Chiwerengero cha maselo omwe akukula mofulumira amatsutsa serum complement-mediated lysis.Kuchulukirachulukira kwakukulu kwa ma cell omwe ali ndi kukula kwapakatikati kumakhudzidwa ndi seramu.Anthu achitatu, omwe sayambiranso kukula atachepetsedwa kuchokera pagawo loyima, amatetezedwa nthawi imodzi ku zowonjezera seramu ndi maantibayotiki.