Zogulitsa

Kuwunika kwa pharmacodynamics kuwonjezera pa pharmacokinetics ndi imodzi mwa njira zopangira chithandizo cha mycophenolate mofetil payekha.Cholinga cha phunziroli chinali kupanga tcheru zamadzimadzi chromatography-tandem mass spectrometry (LC-MS/MS) njira zowunika za pharmacokinetics ndi pharmacodynamics za mycophenolic acid (MPA).Kukhazikika kwa mycophenolic acid glucuronide (MPAG), mycophenolic acid acyl-glucuronide, komanso MPA yosagwirizana ndi MPAG, idatsimikiziridwa, ndipo ntchito ya inosine-5'-monophosphate dehydrogenase idawerengedwa poyesa kuchuluka kwa xanthosine-5'-monophosphate (XMP). ) ndi intracellular adenosine-5'-monophosphate pambuyo pa kukulitsidwa kwa zotumphukira zamagazi a mononuclear cell (PBMC) lysates.Mzere wopanda zitsulo wa Mastro(TM) ndi ma gradient awiri adagwiritsidwa ntchito kukonza malire a XMP mpaka 0.1 μM.M'magulu azachipatala a MPA, mizere yokhotakhota, kulondola kwapakati ndi mkati mwa tsiku ndi kulondola kumakwaniritsa malangizo a US Food and Drug Administration.Magulu a MPA mu hematopoietic stem cell transplant (HSCT) odwala omwe amatsimikiziridwa ndi enzyme assay ndi njira yamakono ya LC-MS / MS inasonyeza kugwirizanitsa bwino (r (2) = 0.95, p <0.001).Mu phunziro ili, timapereka njira zomveka komanso zovomerezeka za LC-MS/MS kuti tiyese mankhwala a pharmacokinetics ndi pharmacodynamics a MPA, omwe ali okhudzidwa mokwanira kuti aone zochepa za PBMC lysates zomwe zimasonkhanitsidwa posakhalitsa HSCT.