6-Chloropurine CAS: 87-42-3 Kuwala chikasu ufa
| Nambala ya Catalog | XD90547 |
| Dzina lazogulitsa | 6-Chloropurine |
| CAS | 87-42-3 |
| Molecular Formula | C5H3ClN4 |
| Kulemera kwa Maselo | 154.557 |
| Zambiri Zosungira | 2 mpaka 8 ° C |
| Harmonize Tariff Code | 2933990090 |
Mafotokozedwe a Zamalonda
| Maonekedwe | Ufa wachikasu wopepuka |
| Kuyesa | 99% |
Tanena posachedwa kuti pyrene nucleotide imayikidwa moyang'anizana ndi malo a abasic, 3'-T ya thymine dimer, ndi maziko omwe sanawonongeke ndi yisiti DNA polymerase eta (pol eta).Chifukwa pyrene ndi molekyulu yopanda polar yopanda mphamvu ya H-bonding, mphamvu zachilendo za kuyika kwa dPMP zimasonyezedwa ndi luso lake lapamwamba la stacking, ndikugogomezera kufunikira kwa maziko a stacking posankha ma nucleotides ndi pol eta.Kuti tifufuze ntchito ya H-bonding ndi base pair geometry posankha ma nucleotides ndi pol eta, tidawona momwe ma nucleotides osinthidwa 2,6-diaminopurine, 2-aminopurine, 6-chloropurine, ndi inosine amathandizira. pangani ma H-bond osiyanasiyana ndi maziko a template kutengera geometry yoyambira.Watson-Crick base pairing akuwoneka kuti ali ndi gawo lofunikira pakusankha ma nucleotide analogues kuti ayikidwe moyang'anizana ndi C ndi T monga zikuwonetseredwa ndi kuchepa kwa magwiridwe antchito ophatikizika ndi kuchepa kwa ma bond a Watson-Crick H-bond komanso kuwonjezeka kwa chiwerengero cha opereka-wopereka ndi ovomereza-kuvomereza kuyanjana.Kusankhidwa kwa nucleotide kulowetsedwa kumakhala kosiyana kwambiri ndi 5'-T kuposa 3'-T ya thymine dimer, mogwirizana ndi ntchito yapitayi yosonyeza kuti 5'-T imagwiridwa mwamphamvu kwambiri kuposa 3'-T.Kuphatikiza apo, kuyika kwa A motsutsana ndi onse a Ts a dimer kumawoneka kuti kumalumikizidwa ndi Watson-Crick base pairing osati ndi Hoogsteen base pairing kutengera kuphatikizika kofananako kwa A ndi 7-deaza-A, komaliza komwe kulibe H- luso lolumikizana pa N7.Kuthekera kwapang'onopang'ono kwa kuyika kwa ma nucleotides omwe amatha kupanga awiriawiri oyambira a Watson-Crick amafanana ndi a chidutswa cha Klenow, pomwe chidutswa cha Klenow chimasankha kwambiri zosagwirizana, malinga ndi kusankha kwake kwakukulu.Zotsatira izi zikugogomezera kufunikira kwa H-bonding ndi Watson-Crick base pair geometry posankha ma nucleotides ndi pol eta ndi Klen Ow fragment, komanso gawo locheperako la kusankha mawonekedwe poyika ndi pol eta chifukwa chotseguka komanso chocheperako. malo osagwira ntchito.
