Mabakiteriya a Colonic, owonetseredwa ndi Bacteroides thetaiotaomicron, amagwira ntchito yofunika kwambiri posunga thanzi laumunthu pogwiritsa ntchito mabanja akuluakulu a glycoside hydrolases (GHs) kuti agwiritse ntchito ma polysaccharides a zakudya ndi ma glycans monga zakudya.Kukula kwa banja la GH koteroko kumasonyezedwa ndi 23 banja la GH92 glycosidases lolembedwa ndi B. thetaiotaomicron genome.Apa tikuwonetsa kuti awa ndi ma alpha-mannosidase omwe amagwira ntchito pogwiritsa ntchito njira imodzi yosamuka kuti agwiritse ntchito N-glycans.Mapangidwe atatu amtundu wa mannosidase awiri a GH92 amatanthawuza banja la mapuloteni awiri omwe ali ndi malo opangira malo, kupereka chithandizo cha asidi (glutamate) ndi maziko (aspartate) ku hydrolysis mu Ca (2+) - njira yodalira.Mapangidwe amitundu itatu a GH92s ovuta okhala ndi zoletsa amapereka chidziwitso chatsatanetsatane, makina ndi njira yofananira ya catalysis.Ca (2+) imagwira ntchito yofunika kwambiri pothandizira kupotoza mannoside kutali ndi mawonekedwe ake apansi (4) C (1) mpando wopita kugawo la kusintha.
