4-Nitrophenyl-alpha-D-galactopyranoside CAS: 7493-95-0 99%
| Nambala ya Catalog | XD900018 |
| Dzina lazogulitsa | 4-Nitrophenyl-alpha-D-galactopyranoside |
| CAS | 7493-95-0 |
| Molecular Formula | C12H15NO8 |
| Kulemera kwa Maselo | 301.25 |
| Zambiri Zosungira | -15 mpaka -20 ° C |
| Harmonize Tariff Code | 29400000 |
Mafotokozedwe a Zamalonda
| Maonekedwe | White mpaka Orange mpaka Green ufa mpaka kristalo |
| Kuyesa | 99% |
4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) ndi gawo lapansi lochita kupanga la 4-nitrophenyl (pNP) glycopyranoside pozindikira ntchito ya α-galactosidase.Kuchuluka kwa pNP yotulutsidwa kunawonjezeka kwambiri pamene 4-Nitrophenyl α-D-galactopyranoside inagwiritsidwa ntchito ngati gawo lapansi.
Kugwiritsa ntchito4-nitorophenol (pNP) -glycopyranosidesaspseudosubstrates, kuchuluka kwamasulidwepNPkuchokera ku4-Nitrophenylα-D-galactopyranosidendikukwezeka kwambirikuchokerakwaatherpNP-glycopyranosidesatpH4.0pamenepamenepanalibechinthuchinapezekapochinthuchimodzichinapezekapochinthuchochepa-panapang'onopang'ono. nyl (pNP) glycopyranosidesasartificial substrates amasonyeza kutiomasulidwa galactoseresidues kuchokera ku4-Nitrophenylα-D-galactopyranosidemainlyatpH4.0, iethepHlevelwhere yieldthresholdisdecreasedatmost.
Gawo laling'ono la α-D-galactosidase (α-D-Galactosidase).Amamanga ku E. coli lactose chonyamulira mapuloteni.Yapakatikati ya α-D-galactose.
Njira yofulumira yowunika ntchito ya lytic ya antimicrobial agents motsutsana ndi yisiti ndi bowa yapangidwa.Kuyesaku kumatengera kutulutsa kwa intracellular enzyme, maltase (alpha-glucosidase).Ntchito yotulutsidwa ya maltase idayesedwa colorimetrically ndi kupanga p-nitrophenol kuchokera ku p-nitrophenyl-alpha-D-glucopyranoside (PNPG).Ntchito ya lytic yamitundu yosiyanasiyana ya antimicrobial idayesedwa motsutsana ndi ma cell a yisiti kapena ma spores omwe amamera a fungus.Lytic anti-yeast zochita zitha kudziwika mkati mwa mphindi 20 zokulitsidwa pa 30 digiri C motsutsana ndi Saccharomyces cerevisiae, Candida albicans, ndi Cryptococcus neoformans.Ntchito ya Lytic yolimbana ndi mafangasi idawoneka pambuyo pa maola 2 a makulitsidwe pa 30 digiri C motsutsana ndi kumera mbewu za Aspergillus niger ndi Botrytis cinerea.Maselo onse a yisiti kapena bowa sanapangitse PNPG yokwanira hydrolyze mkati mwa 3 h pa 30 ° C kuti apangitse kusintha kwamtundu.Kuchita kwa ma enzymes (mwachitsanzo, Lyticase), maantibayotiki (mwachitsanzo, Amphotericin B), ndi mtundu wa mabakiteriya omwe amapanga mabakiteriya adapezeka pogwiritsa ntchito kuyesako.Kuyesa kotsutsa yisiti kwasinthidwa kukhala mawonekedwe a 96-well microtiter.Mayesero onsewa adapereka chidziwitso chofulumira, chodziwika bwino, komanso chodziwika bwino cha lytic anti-yeast ndi anti-fungal ntchito.
