Zogulitsa

1.Tinasanthula ndi kuyerekezera zotsatira za ma H1-antihistamines pa stimulated oxidative kuphulika pa owonjezera-ndi intracellular mlingo wa olekanitsidwa ndi stimulated human polymorphonuclear leukocytes.Oxidative kupasuka kwa akutali anthu neutrophils anaphunzira pogwiritsa ntchito luminol ndi isoluminol kupititsa patsogolo chemiluminescence. Udindo wa potency wa H1-antihistamines kuti uchepe chemiluminescence unayesedwa kunja kwa maselo: dithiaden> loratadine> chlorpheniramine> brompheniramine> pheniramine ndi pa intracellular malo: loratadine> dithiaden. - ndi okhudza maselo ambiri malo a akutali analimbikitsa anthu neutrophils.

2.Chemiluminescence systems zowonjezeredwa ndi isoluminol kapena luminol pamodzi ndi peroxidase ndi njira zodziwika bwino zodziwira mitundu ya okosijeni yowonongeka (ROS) yopangidwa ndi phagocyte NADPH oxidase.Magawo awiri okulitsa ndi ofanana kwambiri, amasiyana pokha pomwe gulu la amino lili mu mphete yonunkhira ya mamolekyu.Kusiyanaku kumapangitsa isoluminol kukhala molekyu yocheperako ya lipophilic yomwe simatha kulowa mu nembanemba yachilengedwe.Kugwiritsiridwa ntchito kwa isoluminol kumangokhala ku maphunziro okhudza katulutsidwe wa oxygen metabolites.Mu phunziro ili tikuwonetsa kuti ma peptides opangidwa kuchokera ku N-terminal domain ya calcium-regulated protein annexin AI amasokoneza kuzindikira kwa ma radicals mu isoluminol-amplified, koma osati mu luminol-amplified, system.Ma peptides opangidwa ndi annexin AI amachepetsa kutuluka kwa kuwala ndi isoluminol yokondwa ndi superoxide ndi horseradish peroxidase (HRP) mu foryl-methionyl-leucyl-phenylalanine- ndi phorbol my ristate acetate-stimulated cells, komanso ndi hydrogen peroxide ndi HRP.Njira yeniyeni yoletsa kuletsa sikudziwika.Zotsatira zomwe zaperekedwa zikuwonetsa kuti kuchepa kwa ma cell kuyankha komwe kumapezeka ndi isoluminol-amplified chemiluminescence kuyenera kutsimikiziridwa ndi njira ina yodziwira kutulutsa kwa superoxide anions ndi hydrogen peroxide.