Zogulitsa

1.Tinaphunzira za inhibitory zotsatira za isorhamnetin pa bowa tyrosinase ndi inhibition kinetics ndi computational simulation.Isorhamnetin inhibited reversibly inhibited tyrosinase m'njira yosakanikirana pa Ki=0.235±0.013 mM.Miyezo ya intrinsic ndi 1-anilinonaphthalene-8-sulfonate(ANS)-binding fluorescence inasonyeza kuti isorhamnetin sinapangitse kusintha kwakukulu mu kapangidwe kapamwamba ka tyrosinase.Kuti timvetsetse momwe kachitidwe kameneka kamagwirira ntchito, ma kinetics adawerengedwa kudzera mumiyezo yanthawi yayitali komanso machitidwe a gawo lapansi mosalekeza.Zotsatirazi zikuwonetsa kuti kusagwira ntchito komwe kumayambitsidwa ndi isorhamnetin kunali koyambirira kochita ndi njira za biphasic.Kuti tidziwe zambiri, tidayerekeza docking pakati pa tyrosinase ndi isorhamnetin.Kuyerekezera kunapambana (kumanga mphamvu kwa Dock6.3: -32.58 kcal / mol, kwa AutoDock4.2: -5.66 kcal / mol, ndi Fred2.2: -48.86 kcal / mol), kutanthauza kuti isorhamnetin imagwirizana ndi zotsalira zingapo, monga monga HIS244 ndi MET280.Njira iyi yolumikizirana ndi predic ting tyrosinase kuphatikiza ndi kinetics yozikidwa pa flavanone pawiri ikhoza kukhala yothandiza pakuwunika zomwe zitha kukhala zachilengedwe za tyrosinase inhibitors.

2. Acid unfolding pathway of conalbumin (CA), monomeric glycoprotein yochokera ku dzira loyera la nkhuku, yafufuzidwa pogwiritsa ntchito ma CD akutali komanso pafupi ndi UV CD, intrinsic fluorescence emission, extrinsic fluorescence probe 1-anilino-8-napthalene sulfonate (Anilino-8-napthalene sulfonate) ndi dynamic light scattering (DLS).Timawona kusintha kodalira pH pamapangidwe achiwiri ndi apamwamba a CA.Ili ndi mawonekedwe achiwiri a α-helical pa pH 4.0 koma mawonekedwe otayika pa pH 3.0.CA inalipo yokha ngati dziko losungunuka lisanasungunuke ndi dziko losungunuka losungunuka mu pH 4.0 ndi pH 3.0, motsatira.Zotsatira za pH pakusintha ndi kutentha kwa CA zimalozera kukana kwake kutentha pa pH ya ndale.Zotsatira za DLS zikuwonetsa kuti dziko la MG linalipo ngati mawonekedwe amadzimadzi okhala ndi hydrodynamic radii ya 4.7 nm.Kuzimitsidwa kwa tryptophan fluorescence ndi acrylamide kunatsimikiziranso kudzikundikira kwa dziko lapakatikati, lotseguka pang'ono, pakati pa mayiko omwe ali mbadwa ndi omwe sanasinthe.